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Can J Microbiol


Title:A review of techniques for detecting Huanglongbing (greening) in citrus
Author(s):Arredondo Valdes R; Delgado Ortiz JC; Beltran Beache M; Anguiano Cabello J; Cerna Chavez E; Rodriguez Pagaza Y; Ochoa Fuentes YM;
Address:"a Universidad Autonoma Agraria Antonio Narro, Department of Agricultural Parasitology, Buenavista, Saltillo, Coahuila, C.P. 25315, Mexico. b Catedratica CONACYT-Universidad Autonoma Agraria Antonio Narro, Department of Agricultural Parasitology, Buenavista, Saltillo, Coahuila, C.P. 25315, Mexico"
Journal Title:Can J Microbiol
Year:2016
Volume:20160617
Issue:10
Page Number:803 - 811
DOI: 10.1139/cjm-2016-0022
ISSN/ISBN:1480-3275 (Electronic) 0008-4166 (Linking)
Abstract:"Huanglongbing (HLB) is the most destructive disease of citrus worldwide. Monitoring of health and detection of diseases in trees is critical for sustainable agriculture. HLB symptoms are virtually the same wherever the disease occurs. The disease is caused by Candidatus Liberibacter spp., vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Electron microscopy was the first technique used for HLB detection. Nowadays, scientists are working on the development of new techniques for a rapid HLB detection, as there is no sensor commercially accessible for real-time assessment of health conditions in trees. Currently, the most widely used mechanism for monitoring HLB is exploration, which is an expensive, labor-intensive, and time-consuming process. Molecular techniques such as polymerase chain reaction are used for the identification of HLB disease, which requires detailed sampling and processing procedures. Furthermore, investigations are ongoing in spectroscopic and imaging techniques, profiling of plant volatile organic compounds, and isothermal amplification. This study recognizes the need for developing a rapid, cost-effective, and reliable health-monitoring sensor that would facilitate advancements in HLB disease detection. This paper compares the benefits and limitations of these potential methods for HLB detection"
Keywords:Animals Biomarkers/metabolism Citrus/metabolism/*microbiology Molecular Typing Plant Diseases/*microbiology Polymerase Chain Reaction Rhizobiaceae/*genetics Hlb Mdj arbres detection health monitoring sante surveillance trees;
Notes:"MedlineArredondo Valdes, Roberto Delgado Ortiz, Juan C Beltran Beache, Mariana Anguiano Cabello, Julia Cerna Chavez, Ernesto Rodriguez Pagaza, Yolanda Ochoa Fuentes, Yisa M eng Review Canada 2016/09/04 Can J Microbiol. 2016 Oct; 62(10):803-811. doi: 10.1139/cjm-2016-0022. Epub 2016 Jun 17"

 
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