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Exp Appl Acarol

Title:		Expression and functional analysis of Niemann-Pick C2 gene in Phytoseiulus persimilis
Author(s):		Zhou H; Yan H; Wang E; Zhang B; Xu X;
Address:		"Lab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. Lab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. wangendong@caas.cn. Key Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, 100193, Beijing, China. wangendong@caas.cn. Lab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. zhangbo05@caas.cn. Key Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, 100193, Beijing, China. zhangbo05@caas.cn. Lab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. xuxuenong@caas.cn. Key Laboratory of Natural Enemies Insects, Ministry of Agriculture and Rural Affairs, 100193, Beijing, China. xuxuenong@caas.cn"
Journal Title:		Exp Appl Acarol
Year:		2023
Volume:		20230315
Issue:		2
Page Number:		201 - 213
DOI:		10.1007/s10493-023-00781-8
ISSN/ISBN:		1572-9702 (Electronic) 0168-8162 (Linking)
Abstract:		"As a new protein class of semiochemical binding and transporting, Niemann-Pick proteins type C2 (NPC2) in arthropods have received more attentions in recent decade. However, the gene function has not been studied in phytoseiid mites with biocontrol potential. In the current study, we cloned a NPC2 gene PpNPC2a from the transcriptome of Phytoseiulus persimilis Athias-Henriot. By encoding 181 amino acids with a conserved ML domain, PpNPC2a was found a homolog of NPC2-1 in Galendromus occidentalis Nesbitt. We then measured the spatio-temporal expression of PpNPC2a in P. persimilis, and found the highest expression in female adults compared to other stages. Due to the tiny body size of predatory mites, we only examined tissue expressions in two sections: the anterior part (gnathosoma and the first pair of legs) and the posterior part (idiosoma without the first pair of legs). Higher transcription of PpNPC2a was found in the posterior part. To investigate the potential function of PpNPC2a in P. persimilis, we interfered gene expression in female adults by feeding dsRNA, which resulted in a decrease of relative expression by 59.1 and 78.2% after 24 and 72 h, respectively. Compared with the control, dsNPC2a-treated P. persimilis were insensitive to the scent of leaves or plants infested by spider mites, suggesting a role of PpNPC2a in response to plant volatiles. However, the dsNPC2a-interfered mites could still respond to four representative compounds of herbivore-induced plant volatiles, including 4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), methyl salicylate (MeSA), beta-caryophyllene and linalool. In short, our results indicated PpNPC2a may be involved in the chemosensory process of P. persimilis in response to whole-plant volatiles"
Keywords:		Animals *Odorants Plants Pheromones Plant Leaves Predatory Behavior/physiology *Tetranychidae/physiology Behavioral response Olfaction Phytoseiidae Predatory mite RNA interference;
Notes:		"MedlineZhou, Hongxu Yan, Hong Wang, Endong Zhang, Bo Xu, Xuenong eng BAIC01-2022/Beijing Agriculture Innovation Consortium/ No. 32070402/National Natural Science Foundation of China/ No. 6222052/Beijing Natural Science Foundation/ Netherlands 2023/03/16 Exp Appl Acarol. 2023 Feb; 89(2):201-213. doi: 10.1007/s10493-023-00781-8. Epub 2023 Mar 15"