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Pest Manag Sci

Title:		Molecular mechanism of sex pheromone perception in male Mythimna loreyi revealed by in vitro system
Author(s):		Zhang YY; Bai TF; Guo JM; Wei ZQ; Liu SR; He Y; Ye JJ; Yan Q; Zhang J; Dong SL;
Address:		"Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education / College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China"
Journal Title:		Pest Manag Sci
Year:		2023
Volume:		20231001
Issue:
Page Number:		-
DOI:		10.1002/ps.7806
ISSN/ISBN:		1526-4998 (Electronic) 1526-498X (Linking)
Abstract:		"BACKGROUND: Mythimna loreyi is an important agricultural pest with a sensitive sex pheromone communication system. To clarify the pheromone binding proteins (PBPs) and pheromone receptors (PRs) involved in sex pheromone perception is important for both understanding the molecular olfactory mechanism and developing new pest control strategy in M. loreyi. RESULTS: First, the electroantennogram (EAG) assay showed that male M. loreyi displayed highest response to the major sex pheromone component Z9-14:Ac, and higher response to two minor component Z7-12:Ac and Z11-16:Ac. Second, the fluorescence competition binding assay showed that PBP1 bound all three pheromones and other tested compounds with high or moderate affinity, while PBP2 and PBP3 each bound only one pheromone component and few other compounds. Third, functional study using the Xenopus oocyte system demonstrated that, of the 6 candidate PRs, PR2 was weakly sensitive to the major pheromone Z9-14:Ac, but strongly to pheromone analog Z9-14:OH; PR3 was strongly and specifically sensitive to a minor component Z7-12:Ac; PR4 and OR33 were both weakly respond to another minor component Z11-16:Ac. Finally, phylogenetic relationship and ligand profiles of PRs were compared among 6 species from two closely related genera Mythimna and Spodoptera, suggesting functional shifts of M. loreyi PRs toward Spodoptera PRs. CONCLUSION: Functional differentiation were revealed among three PBPs and 6 PRs in sex pheromone perception, laying an important basis for understanding the molecular mechanism of sex pheromone perception and for developing new control strategy in M. loreyi. This article is protected by copyright. All rights reserved"
Keywords:		Mythimna loreyi functional differentiation pheromone binding protein pheromone receptor sex pheromone;
Notes:		"PublisherZhang, Yun-Ying Bai, Teng-Fei Guo, Jin-Meng Wei, Zhi-Qiang Liu, Si-Ruo He, Yu Ye, Jing-Jing Yan, Qi Zhang, Jin Dong, Shuang-Lin eng England 2023/10/02 Pest Manag Sci. 2023 Oct 1. doi: 10.1002/ps.7806"