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Gen Comp Endocrinol

Title:		Cloning and olfactory expression of progestin receptors in the Chinese black sleeper Bostrichthys sinensis
Author(s):		Zhang YT; Liu DT; Zhu Y; Chen SX; Hong WS;
Address:		"College of Ocean and Earth Sciences, Xiamen University, Fujian 361005, PR China. College of Ocean and Earth Sciences, Xiamen University, Fujian 361005, PR China; Department of Biology, Howell Science Complex, East Carolina University, 1000 E, 5th Street, Greenville, NC 27858-4553, USA. College of Ocean and Earth Sciences, Xiamen University, Fujian 361005, PR China. Electronic address: chenshixi@xmu.edu.cn. College of Ocean and Earth Sciences, Xiamen University, Fujian 361005, PR China. Electronic address: wshong@xmu.edu.cn"
Journal Title:		Gen Comp Endocrinol
Year:		2016
Volume:		20160324
Issue:
Page Number:		87 - 102
DOI:		10.1016/j.ygcen.2016.03.029
ISSN/ISBN:		1095-6840 (Electronic) 0016-6480 (Print) 0016-6480 (Linking)
Abstract:		"Our previous studies suggested that 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), an oocyte maturation inducing progestin, also acts as a sex pheromone in Chinese black sleeper Bostrichthys sinensis, a fish species that inhabits intertidal zones and mates and spawns inside a muddy burrow. The electro-olfactogram response to DHP increased during the breeding season. In the present study, we cloned the cDNAs of the nine progestin receptors (pgr, paqr5, 6, 7(a, b), 8, 9, pgrmc1, 2) from B. sinensis, analyzed their tissue distribution, and determined the expression in the olfactory rosette during the reproductive cycle in female and male fish. The deduced amino acid sequences of the nine progestin receptors share high sequence identities with those of other fish species and relatively lower homology with their mammalian counterparts, and phylogenetic analyses classified the nine B. sinensis progestin receptors into their respective progestin receptor groups. Tissue distribution of B. sinensis progestin receptors showed differential expression patterns, but all these nine genes were expressed in the olfactory rosette. Interestingly, paqr5 mRNA was found in the intermediate and basal parts of the olfactory epithelium but not in the central core using in situ hybridization, and its expression level was the highest in the olfactory rosette among the tissues examined. These results suggested Paqr5 may have an important role for transmitting progestin signaling in the olfactory system. The expression levels of paqr7a and paqr7b, pgr and pgrmc2 mRNA peaked around the mid meiotic stage, and that of paqr8 peaked at late meiotic stage in the olfactory rosette in males, while the olfactory expression of paqr5 decreased gradually as spermatogenesis progressed. In contrast, the expression of the progestin receptors did not change significantly during the development of the ovary in the olfactory rosette in females, except that of pgr. Interestingly, the changes of paqr8 expression in the olfactory rosette in males mirrored the changes of plasma DHP levels in females during the reproductive cycle, suggesting the Paqr8 may also be important for deciphering progestin signaling released by female. To our knowledge, this is the first time to demonstrate the presence of all known progestin receptors in a teleost olfactory rosette, and to show different expressions between the males and females during the reproductive cycle. This study provides the first evidence on changes of all purported progestin receptors during a reproductive cycle in teleost olfactory rosette, and suggests that distinct olfactory sensitivities to DHP may be due to the changes and compositions of each progestin receptor in B. sinensis"
Keywords:		"Animals Cloning, Molecular Female Fishes/genetics/*metabolism Male Ovary/metabolism *Phylogeny Progestins/metabolism RNA, Messenger/genetics Receptors, Progesterone/genetics/*metabolism Reproduction/physiology Spermatogenesis 17alpha, 20beta-Dihydroxy-4-pr;"
Notes:		"MedlineZhang, Yu Ting Liu, Dong Teng Zhu, Yong Chen, Shi Xi Hong, Wan Shu eng R15 GM100461/GM/NIGMS NIH HHS/ 2016/03/29 Gen Comp Endocrinol. 2016 May 1; 230-231:87-102. doi: 10.1016/j.ygcen.2016.03.029. Epub 2016 Mar 24"