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New Phytol

Title:		Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation
Author(s):		Zhang Y; Li ZX; Yu XD; Fan J; Pickett JA; Jones HD; Zhou JJ; Birkett MA; Caulfield J; Napier JA; Zhao GY; Cheng XG; Shi Y; Bruce TJA; Xia LQ;
Address:		"The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. Tobacco Research Institute, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, 11 Keyuanjing 4 Road, Laoshan District, Qingdao, 266101, China. Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China"
Journal Title:		New Phytol
Year:		2015
Volume:		20150130
Issue:		3
Page Number:		1101 - 1115
DOI:		10.1111/nph.13302
ISSN/ISBN:		1469-8137 (Electronic) 0028-646X (Linking)
Abstract:		"Aphids are important pests of wheat (Triticum aestivum) that affect crop production globally. Herbivore-induced emission of sesquiterpenes can repel pests, and farnesyl pyrophosphate synthase (FPS) is a key enzyme involved in sesquiterpene biosynthesis. However, fps orthologues in wheat and their functional roles in sesquiterpene synthesis and defence against aphid infestation are unknown. Here, two fps isoforms, Tafps1 and Tafps2, were identified in wheat. Quantitative real-time polymerase chain reaction (qRT-PCR) and in vitro catalytic activity analyses were conducted to investigate expression patterns and activity. Heterologous expression of these isoforms in Arabidopsis thaliana, virus-induced gene silencing (VIGS) in wheat and aphid behavioural assays were performed to understand the functional roles of these two isoforms. We demonstrated that Tafps1 and Tafps2 played different roles in induced responses to aphid infestation and in sesquiterpene synthesis. Heterologous expression in A. thaliana resulted in repulsion of the peach aphid (Myzus persicae). Wheat plants with these two isoforms transiently silenced were significantly attractive to grain aphid (Sitobion avenae). Our results provide new insights into induced defence against aphid herbivory in wheat, in particular, the different roles of the two Tafps isoforms in both sesquiterpene biosynthesis and defence against aphid infestation"
Keywords:		"Amino Acid Sequence Animals Aphids/*physiology Arabidopsis/genetics/metabolism Gene Silencing Geranyltranstransferase/*chemistry/genetics Herbivory Host-Parasite Interactions/genetics Isoenzymes/chemistry/genetics Molecular Sequence Data Plants, Genetical;"
Notes:		"MedlineZhang, Yan Li, Zhi-Xia Yu, Xiu-Dao Fan, Jia Pickett, John A Jones, Huw D Zhou, Jing-Jiang Birkett, Michael A Caulfield, John Napier, Johnathan A Zhao, Guang-Yao Cheng, Xian-Guo Shi, Yi Bruce, Toby J A Xia, Lan-Qin eng BB/G004781/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BBS/E/C/00004158/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BBS/E/C/00004975/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2015/02/04 New Phytol. 2015 May; 206(3):1101-1115. doi: 10.1111/nph.13302. Epub 2015 Jan 30"