| Title: | Odor fingerprinting of Listeria monocytogenes recognized by SPME-GC-MS and E-nose |
| Author(s): | Yu YX; Sun XH; Liu Y; Pan YJ; Zhao Y; |
| Address: | "College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, People's Republic of China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage & Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, People's Republic of China" |
| ISSN/ISBN: | 1480-3275 (Electronic) 0008-4166 (Linking) |
| Abstract: | "Microorganisms can produce species-specific microbial volatile organic compounds (MVOCs), or odor compounds, which can be characterized by odor fingerprinting. The objective of this study was to characterize the odor fingerprint of Listeria monocytogenes. Solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) and electronic nose (E-nose) were used to recognize the MVOCs of L. monocytogenes in pure culture medium. The main MVOCs of L. monocytogenes were identified by SPME-GC-MS analysis as alcohols, aldehydes, ketones, alkanes, and heterocyclics, among which the relative peak area of one compound, 3-hydroxy-2-butanone, increased along with the growth of L. monocytogenes. The odor fingerprint of L. monocytogenes at different growth stages could be clearly discriminated by E-nose. In addition, E-nose signals had a very good linear relationship with the concentration of this bacterium (R(2) = 0.9937). Our study may help to establish the analysis of the odor fingerprint of microorganisms as a potential routine method in microbiology" |
| Keywords: | *Electronic Nose Gas Chromatography-Mass Spectrometry/*methods Listeria monocytogenes/*metabolism *Odorants Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis Covm E-nose Listeria monocytogenes MVOCs Spme-gc-ms detection empreinte o; |
| Notes: | "MedlineYu, Yong-xin Sun, Xiao-hong Liu, Yuan Pan, Ying-jie Zhao, Yong eng Research Support, Non-U.S. Gov't Canada 2015/04/08 Can J Microbiol. 2015 May; 61(5):367-72. doi: 10.1139/cjm-2014-0652. Epub 2014 Dec 8" |
