| Title: | Identification and functional characterization of yeast zeta-COP |
| Author(s): | Yamazaki S; Harashima S; Sakaguchi M; Mihara K; |
| Address: | "Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka" |
| DOI: | 10.1093/oxfordjournals.jbchem.a021574 |
| ISSN/ISBN: | 0021-924X (Print) 0021-924X (Linking) |
| Abstract: | "Coatomer, the cytosolic protein complex, consists of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) and is involved in vesicle trafficking early in the secretory pathway in collaboration with a 20 kDa GTP-binding protein (ARF). In the present study, we have identified a yeast gene which encodes a protein having 39% amino acid sequence identity with bovine zeta-COP. This gene (YZC1 for Yeast Zeta COP) is essential for vegetative growth and the growth defect of delta yzc1 cells was restored by bovine zeta-COP cDNA. We isolated a temperature-sensitive mutant of YZC1 (yzc1ts) and examined its capacity for both the ER-to-Golgi transport and the double lysine motif (KKXX)-mediated retrograde transport from Golgi to ER. At non-permissive temperature, the yzc1ts cells exhibited a weak defect in the anterograde transport, but a strong defect in the retrograde vesicle transport. We conclude that Yzc1p is a yeast homologue of mammalian zeta-COP and participates mainly in the Golgi-to-ER retrograde transport" |
| Keywords: | "Amino Acid Sequence Animals Biological Transport Carrier Proteins/chemistry/*genetics/*metabolism Cattle Coatomer Protein Endoplasmic Reticulum/genetics/metabolism Fungal Proteins/chemistry/*genetics/*metabolism Genes, Fungal Genetic Complementation Test;" |
| Notes: | "MedlineYamazaki, S Harashima, S Sakaguchi, M Mihara, K eng England 1997/01/01 J Biochem. 1997 Jan; 121(1):8-14. doi: 10.1093/oxfordjournals.jbchem.a021574" |
