| Title: | Niemann-Pick proteins type C2 are identified as olfactory related genes of Pardosa pseudoannulata by transcriptome and expression profile analysis |
| Author(s): | Xiu C; Xiao Y; Zhang S; Bao H; Liu Z; Zhang Y; |
| Address: | "Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: liuzewen@njau.edu.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn" |
| Journal Title: | Comp Biochem Physiol Part D Genomics Proteomics |
| DOI: | 10.1016/j.cbd.2019.01.004 |
| ISSN/ISBN: | 1878-0407 (Electronic) 1744-117X (Linking) |
| Abstract: | "In arthropods, the large majority of studies on olfaction have been mainly focused on insects, whereas little on Arachnida, even though olfaction is very important in arachnid behavior. Pardosa pseudoannulata is one of the most common wandering spiders in rice fields, as the important natural enemy against a range of pests. However, little is known about the potential chemosensory proteins involved in olfactory behavior of these spiders. Niemann-Pick proteins type C2 (NPC2) as a new class of binding and transport proteins for semiochemicals in arthropods especially ticks and mites has received more attention in recent years. In this study, six NPC2s namely PpseNPC1-6 were newly identified in the appendages of P. pseudoannulata based on transcriptome data. A phylogenetic analysis indicated that all of P. pseudoannulata NPC2s were clustered together forming one clade with high posterior probability values. In addition, the sequences shared the same subclade with the NPC2 sequences of ticks and scorpion. The motif-patterns indicated that PpseNPC2-5 had the common pattern with the two-spotted spider mite Tetranychus urticae and the ant Trachymyrmex cornetzi. Furthermore, quantitative real-time PCR (qPCR) measurements were conducted to evaluate the expression profile of these genes in various tissues of P. pseudoannulata. It was found that most NPC2s (PpseNPC2-1, PpseNPC2-2, PpseNPC2-5 and PpseNPC2-6) were highly expressed in adult pedipalps and chelicerae. Owing to the functional olfactory organs in Chelicerata of pedipalps, our results supported a putative role of NPC2s as new odorant carriers in P. pseudoannulata" |
| Keywords: | Animals Arthropod Proteins/genetics Arthropods/*metabolism Carrier Proteins/*metabolism *Gene Expression Profiling Glycoproteins/*metabolism Humans Mites/physiology Smell/*physiology *Transcriptome Vesicular Transport Proteins Expression profile Niemann-P; |
| Notes: | "MedlineXiu, Chunli Xiao, Yong Zhang, Song Bao, Haibo Liu, Zewen Zhang, Yongjun eng Research Support, Non-U.S. Gov't Netherlands 2019/01/23 Comp Biochem Physiol Part D Genomics Proteomics. 2019 Mar; 29:320-329. doi: 10.1016/j.cbd.2019.01.004. Epub 2019 Jan 17" |
