| Title: | "Degradation of pheromone biosynthesis-activating neuropeptide (PBAN) by hemolymph enzymes of the tobacco hornworm, Manduca sexta, and the corn earworm, Helicoverpa zea" |
| Author(s): | Weirich GF; Kochansky JP; Masler EP; Lusby WR; Feldlaufer MF; Raina AK; Svoboda JA; |
| Address: | "Insect Neurobiology and Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705-2350, USA" |
| ISSN/ISBN: | 0014-4754 (Print) 0014-4754 (Linking) |
| Abstract: | "The tritium-labeled bis-norleucine analog of Helicoverpa zea pheromone biosynthesis-activating neuropeptide ([3H]NLPBAN) was incubated in vitro with hemolymph from Manduca sexta or H. zea adult females. The incubations resulted in the formation of several tritium-labeled degradation products. At a [3H]NLPBAN concentration of 0.9 microM the degradation proceeded at a very slow but physiologically plausible rate (2-10 fmol/min/microliters hemolymph). The primary [3H]NLPBAN degradation reaction in M. sexta hemolymph was not inhibited by 20 microM leupeptin, 0.1 mM amastatin, 1 mM EDTA, 1 mM EGTA, 1 mM 1,10-phenanthroline, or 2 mM 4-(2-aminoethyl)benzenesulfonyl fluoride; but secondary reactions may have been affected, as some of the inhibitors changed the radio-HPLC profile of the degradation products. It is concluded that hemolymph of M. sexta and H. zea contains peptidase(s) capable of inactivating circulating PBAN" |
| Keywords: | Amino Acid Sequence Animals Endopeptidases/*metabolism Female Hemolymph/*enzymology Manduca/*enzymology Molecular Sequence Data Moths/*enzymology Neuropeptides/*metabolism Pheromones/metabolism Protease Inhibitors/pharmacology; |
| Notes: | "MedlineWeirich, G F Kochansky, J P Masler, E P Lusby, W R Feldlaufer, M F Raina, A K Svoboda, J A eng Switzerland 1995/09/29 Experientia. 1995 Sep 29; 51(9-10):961-6. doi: 10.1007/BF01921748" |
