| Title: | Cell Surface Display of MerR on Saccharomyces cerevisiae for Biosorption of Mercury |
| Author(s): | Wei Q; Yan J; Chen Y; Zhang L; Wu X; Shang S; Ma S; Xia T; Xue S; Zhang H; |
| Address: | "College of Life Science, Qufu Normal University, Jingxuan West Street No. 57, Qufu, 273165, Shandong, China. College of Marine Life Sciences, Ocean University of China, Songling Road No. 238, Laoshan District, Qingdao, 266100, Shandong, China. College of Life Science, Qufu Normal University, Jingxuan West Street No. 57, Qufu, 273165, Shandong, China. zhanghonghai67@126.com" |
| DOI: | 10.1007/s12033-017-0039-2 |
| ISSN/ISBN: | 1559-0305 (Electronic) 1073-6085 (Linking) |
| Abstract: | "The metalloregulatory protein MerR which plays important roles in mer operon system exhibits high affinity and selectivity toward mercury (II) (Hg(2+)). In order to improve the adsorption ability of Saccharomyces cerevisiae for Hg(2+), MerR was displayed on the surface of S. cerevisiae for the first time with an alpha-agglutinin-based display system in this study. The merR gene was synthesized after being optimized and added restriction endonuclease sites EcoR I and Mlu I. The display of MerR was indirectly confirmed by the enhanced adsorption ability of S. cerevisiae for Hg(2+) and colony PCR. The hydride generation atomic absorption spectrometry was applied to measure the Hg(2+) content in water. The engineered yeast strain not only showed higher tolerance to Hg, but also their adsorption ability was much higher than that of origin and control strains. The engineered yeast could adsorb Hg(2+) under a wide range of pH levels, and it could also adsorb Hg(2+) effectively with Cd(2+) and Cu(2+) coexistence. Furthermore, the engineered yeast strain could adsorb ultra-trace Hg(2+) effectively. The results above showed that the surface-engineered yeast strain could adsorb Hg(2+) under complex environmental conditions and could be used for the biosorption and bioremediation of environmental Hg contaminants" |
| Keywords: | Adsorption Bacterial Proteins/genetics/*metabolism Cadmium/pharmacology Copper/pharmacology DNA-Binding Proteins/genetics/*metabolism Hydrogen-Ion Concentration Mating Factor/genetics/metabolism Mercury/metabolism/*pharmacokinetics Plasmids/genetics Recom; |
| Notes: | "MedlineWei, Qinguo Yan, Jiakuo Chen, Yao Zhang, Lei Wu, Xiaoyang Shang, Shuai Ma, Shisheng Xia, Tian Xue, Shuyu Zhang, Honghai eng 201404420/the Special Fund for Forest Scientific Research in the Public Welfare/ 2013GSF11707/the Science and Technology Program for Colleges and Universities of Shandong Province/ 31672313/the National Natural Science Fund of China/ 31372220/the National Natural Science Fund of China/ Switzerland 2017/11/13 Mol Biotechnol. 2018 Jan; 60(1):12-20. doi: 10.1007/s12033-017-0039-2" |
