« Previous AbstractIdentification of a new pheromone-binding protein in the antennae of a geometrid species and preparation of its antibody to analyze the antennal proteins of moths secreting type II sex pheromone components    Next AbstractBoth BAT1 and ARO8 are responsible for unpleasant odor generation in halo-tolerant yeast Zygosaccharomyces rouxii »

Front Endocrinol (Lausanne)

Title:		"Biosynthetic Pathway for Sex Pheromone Components Produced in a Plusiinae Moth, Plusia festucae"
Author(s):		Watanabe H; Matsui A; Inomata S; Yamamoto M; Ando T;
Address:		"Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology Koganei, Tokyo, Japan"
Journal Title:		Front Endocrinol (Lausanne)
Year:		2011
Volume:		20111114
Issue:
Page Number:		74 -
DOI:		10.3389/fendo.2011.00074
ISSN/ISBN:		1664-2392 (Electronic) 1664-2392 (Linking)
Abstract:		"While many Plusiinae species commonly secrete (Z)-7-dodecenyl acetate (Z7-12:OAc) as a key pheromone component, female moths of the rice looper (Plusia festucae) exceptionally utilize (Z)-5-dodecenyl acetate (Z5-12:OAc) to communicate with their partners. GC-MS analysis of methyl esters derived from fatty acids included in the pheromone gland of P. festucae showed a series of esters monounsaturated at the omega7-position, i.e., (Z)-5-dodecenoate, (Z)-7-tetradecenoate, (Z)-9-hexadecenoate (Z9-16:Me), and (Z)-11-octadecenoate (Z11-18:Me). By topical application of D(3)-labled palmitic acid (16:Acid) and stearic acid (18:Acid) to the pheromone glands, similar amounts of D(3)-Z5-12:OAc were detected. The glands treated with D(13)-labeled monoenoic acids (Z9-16:Acid and Z11-18:Acid), which were custom-made by utilizing an acetylene coupling reaction with D(13)-1-bromohexane, also produced similar amounts of D(13)-Z5-12:OAc. These results suggested that Z5-12:OAc was biosynthesized by omega7-desaturase with low substrate specificity, which could introduce a double bond at the 9-position of a 16:Acid derivative and the 11-position of an 18:Acid derivative. Additional experiments with the glands pretreated with an inhibitor of chain elongation supported this speculation. Furthermore, a comparative study with another Plusiinae species (Chrysodeixis eriosoma) secreting Z7-12:OAc indicated that the beta-oxidation systems of P. festucae and C. eriosoma were different"
Keywords:		Lepidoptera Noctuidae incorporation of deuterated precursors monoenyl fatty acid pheromone biosynthesis omega7-desaturation;
Notes:		"PubMed-not-MEDLINEWatanabe, Hayaki Matsui, Aya Inomata, Sin-Ichi Yamamoto, Masanobu Ando, Tetsu eng Switzerland 2011/01/01 Front Endocrinol (Lausanne). 2011 Nov 14; 2:74. doi: 10.3389/fendo.2011.00074. eCollection 2011"