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Mol Reprod Dev

Title:		Lipopolysaccharide-induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro-inflammatory cytokines and miR-187
Author(s):		Wang Y; Zhang JJ; Yang WR; Luo HY; Zhang JH; Wang XZ;
Address:		"Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China. College of Resource and Environment, Southwest University, Beibei, Chongqing, P. R. China"
Journal Title:		Mol Reprod Dev
Year:		2015
Volume:		20150825
Issue:		11
Page Number:		880 - 891
DOI:		10.1002/mrd.22534
ISSN/ISBN:		1098-2795 (Electronic) 1040-452X (Linking)
Abstract:		"Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells in a time- and dose-dependent manner; enhanced the production of pro-inflammatory cytokines including tumor necrosis factor alpha (TNFA), interleukin-1beta (IL1B), nitric oxide (NO), and transforming growth factor-beta (TGFB); and increased the expression of FASLG in a dose-dependent manner. While 10 mug/ml LPS enhanced the expression of FASLG, reduced cell cycle progression, and impaired the ultrastructure of Sertoli cells, this dose did not induce apoptosis. LPS also had no effect on the activity or expression of matrix metalloproteinases 2 or 9 (MMP2 or MMP9). In contrast, the expression of ssc-miR-187 increased following LPS challenge, and inhibition of ssc-miR-187 blocked LPS-induced expression of FASLG. Our results therefore suggest that LPS reduces the viability of and enhances FASLG expression in cultured, immature boar Sertoli cells through elevated secretion of TNFA, IL1B, NO, and TGFB as well as through the regulation of ssc-miR-187 potency"
Keywords:		"Animals Cells, Cultured Cytokines/*biosynthesis Fas Ligand Protein/*biosynthesis Gene Expression Regulation/*drug effects Lipopolysaccharides/*pharmacology Male MicroRNAs/*biosynthesis Sertoli Cells/cytology/*metabolism Swine;"
Notes:		"MedlineWang, Yi Zhang, Jiao-Jiao Yang, Wei-Rong Luo, Hong-Yan Zhang, Jia-Hua Wang, Xian-Zhong eng Research Support, Non-U.S. Gov't 2015/08/11 Mol Reprod Dev. 2015 Nov; 82(11):880-91. doi: 10.1002/mrd.22534. Epub 2015 Aug 25"