| Title: | "The analysis of volatile organic compounds biomarkers for lung cancer in exhaled breath, tissues and cell lines" |
| Author(s): | Wang Y; Hu Y; Wang D; Yu K; Wang L; Zou Y; Zhao C; Zhang X; Wang P; Ying K; |
| Address: | "Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, China" |
| ISSN/ISBN: | 1875-8592 (Electronic) 1574-0153 (Linking) |
| Abstract: | "BACKGROUND: Volatile organic compounds (VOCs) biomarkers in breath provide a novel, noninvasive and quick approach to diagnosis lung cancer. The aim of the proposed study was to investigate the VOCs biomarkers in exhaled breath for lung cancer. METHOD: The VOCs in exhaled breath of 88 lung cancer patients, 70 lung benign disease and 85 healthy people were analyzed by Solid Phase Micro Extraction - Gas Chromatography Mass Spectrometry (SPME-GCMS). Three types of lung cancer cells and 18 lung cancer patients' tissues were cultured in vitro. The VOCs in the headspace of these cultivations were analyzed as an evidence of production mechanism of the VOCs in breath. Three lung cancer diagnosis models were constructed respectively in exhaled breath samples using Linear Discriminant Analysis (LDA). Leave one out cross validation was employed to evaluate these models. RESULTS: 23 VOCs, whose areas under curve (AUC) of receiver operating characteristic (ROC) > 0.60 and p < 0.01, were confirmed as the VOCs biomarkers for lung cancer. Three diagnostic models based on 23 VOCs could easily discriminate lung cancer patients from controls with 96.47% sensitivity and 97.47% specificity. However, the discrimination between early stage and later stage lung cancer was not very obvious" |
| Keywords: | "Biomarkers, Tumor/*analysis/metabolism Breath Tests/instrumentation/methods Case-Control Studies Cell Line, Tumor Exhalation Female Humans Lung Neoplasms/diagnosis/*metabolism Male Middle Aged Volatile Organic Compounds/*analysis/metabolism;" |
| Notes: | "MedlineWang, Yishan Hu, Yanjie Wang, Di Yu, Kai Wang, Ling Zou, Yingchang Zhao, Cong Zhang, Xuanlang Wang, Ping Ying, Kejing eng Research Support, Non-U.S. Gov't Netherlands 2012/11/13 Cancer Biomark. 2012; 11(4):129-37. doi: 10.3233/CBM-2012-00270" |
