| Title: | Dexamethasone enhances glucose uptake by SGLT1 and GLUT1 and boosts ATP generation through the PPP-TCA cycle in bovine neutrophils |
| Author(s): | Wang X; Tang M; Zhang Y; Li Y; Mao J; Deng Q; Li S; Jia Z; Du L; |
| Address: | "College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China. Key Laboratory for Prevention and Control of Herbivorous Livestock Perinatal Diseases, Inner Mongolia Minzu University, Tongliao 028000, China. Key Laboratory for Prevention and Control of Herbivorous Livestock Perinatal Diseases, Inner Mongolia Minzu University, Tongliao 028000, China. duliyin2016@163.com" |
| ISSN/ISBN: | 1976-555X (Electronic) 1229-845X (Print) 1229-845X (Linking) |
| Abstract: | "BACKGROUND: Clinical dexamethasone (DEX) treatment or stress in bovines results in extensive physiological changes with prominent hyperglycemia and neutrophils dysfunction. OBJECTIVES: To elucidate the effects of DEX treatment in vivo on cellular energy status and the underlying mechanism in circulating neutrophils. METHODS: We selected eight-month-old male bovines and injected DEX for 3 consecutive days (1 time/d). The levels of glucose, total protein (TP), total cholesterol (TC), and the proinflammatory cytokines interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF)-alpha in blood were examined, and we then detected glycogen and adenosine triphosphate (ATP) content, phosphofructosekinase-1 (PFK1) and glucose-6-phosphate dehydrogenase (G6PDH) activity, glucose transporter (GLUT)1, GLUT4, sodium/glucose cotransporter (SGLT)1 and citrate synthase (CS) protein expression and autophagy levels in circulating neutrophils. RESULTS: DEX injection markedly increased blood glucose, TP and TC levels, the Ca(2+)/P(5+) ratio and the neutrophil/lymphocyte ratio and significantly decreased blood IL-1beta, IL-6 and TNF-alpha levels. Particularly in neutrophils, DEX injection inhibited p65-NFkappaB activation and elevated glycogen and ATP contents and SGLT1, GLUT1 and GR expression while inhibiting PFK1 activity, enhancing G6PDH activity and CS expression and lowering cell autophagy levels. CONCLUSIONS: DEX induced neutrophils glucose uptake by enhancing SGLT1 and GLUT1 expression and the transformation of energy metabolism from glycolysis to pentose phosphate pathway (PPP)-tricarboxylic acid (TCA) cycle. This finding gives us a new perspective on deeper understanding of clinical anti-inflammatory effects of DEX on bovine" |
| Keywords: | *Adenosine Triphosphate Animals Anti-Inflammatory Agents Blood Glucose Cattle Cholesterol Citrate (si)-Synthase Dexamethasone/pharmacology Glucose Transporter Type 1 Glucosephosphate Dehydrogenase Glycogen Interleukin-6 Male *Neutrophils Sodium Tricarboxy; |
| Notes: | "MedlineWang, Xinbo Tang, Mingyu Zhang, Yuming Li, Yansong Mao, Jingdong Deng, Qinghua Li, Shusen Jia, Zhenwei Du, Liyin eng 31760752/National Natural Science Foundation of China/China 31260626/National Natural Science Foundation of China/China 2018LH03009/Natural Science Foundation of Inner Mongolia/China 2019LH03020/Natural Science Foundation of Inner Mongolia/China NJYT-20-B30/Young Scientific and Technological Talents in Inner Mongolia/China Korea (South) 2022/09/30 J Vet Sci. 2022 Sep; 23(5):e76. doi: 10.4142/jvs.22112" |
