| Title: | Degradation of ethyl mercaptan and its major intermediate diethyl disulfide by Pseudomonas sp. strain WL2 |
| Author(s): | Wang X; Wu C; Liu N; Li S; Li W; Chen J; Chen D; |
| Address: | "Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Institute of Industrial Ecology and Environment, College of Chemical and Biological Engineering, Zhejiang University (Yuquan Campus), Hangzhou, 310027, China" |
| Journal Title: | Appl Microbiol Biotechnol |
| DOI: | 10.1007/s00253-014-6208-3 |
| ISSN/ISBN: | 1432-0614 (Electronic) 0175-7598 (Linking) |
| Abstract: | "A Pseudomonas sp. strain WL2 that is able to efficiently metabolize ethyl mercaptan (EM) into diethyl disulfide (DEDS) through enzymatic oxidation was isolated from the activated sludge of a pharmaceutical wastewater plant. One hundred percent removal of 113.5 mg L(-1) EM and 110.3 mg L(-1) DEDS were obtained within 14 and 32 h, respectively. A putative EM degradation pathway that involved the catabolism via DEDS was proposed, which indicated DEDS were further mineralized into carbon dioxide (CO2), bacterial cells, and sulfate (SO4 (2-)) through the transformation of element sulfur and ethyl aldehyde. Degradation kinetics for EM and DEDS with different initial concentrations by strain WL2 were evaluated using Haldane-Andrews model with maximum specific degradation rates of 3.13 and 1.33 g g(-1) h(-1), respectively, and maximum degradation rate constants of 0.522 and 0.175 h(-1) using pseudo-first-order kinetic model were obtained. Results obtained that aerobic degradation of EM by strain WL2 was more efficient than those from previous studies. Substrate range studies of strain WL2 demonstrated its ability to degrade several mercaptans, disulfides, aldehydes, and methanol. All the results obtained highlight the potential of strain WL2 for the use in the biodegradation of volatile organic sulfur compounds (VOSCs)" |
| Keywords: | "Biodegradation, Environmental Carbon Dioxide/metabolism Disulfides/metabolism Kinetics Pseudomonas/genetics/*metabolism RNA, Ribosomal, 16S Sewage/microbiology Sulfates/metabolism Sulfhydryl Compounds/*metabolism Wastewater;" |
| Notes: | "MedlineWang, Xiangqian Wu, Chao Liu, Nan Li, Sujing Li, Wei Chen, Jianmeng Chen, Dongzhi eng Research Support, Non-U.S. Gov't Germany 2014/11/16 Appl Microbiol Biotechnol. 2015 Apr; 99(7):3211-20. doi: 10.1007/s00253-014-6208-3. Epub 2014 Nov 15" |
