| Title: | Antifungal Mechanism of Volatile Organic Compounds Produced by Bacillus subtilis CF-3 on Colletotrichum gloeosporioides Assessed Using Omics Technology |
| Author(s): | Wang K; Qin Z; Wu S; Zhao P; Zhen C; Gao H; |
| Address: | "School of Life Sciences, Shanghai University, Shanghai 200444, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Bacillus subtilis is commonly used as a biocontrol bacterium owing to its strong antifungal activity, broad-spectrum inhibition, and general safety. In this study, the inhibitory effects of volatile organic compounds (VOCs) produced by B. subtilis CF-3 on Colletotrichum gloeosporioides, a major destructive phytopathogen of litchi anthracnose, were analyzed using proteomics and transcriptomics. Differentially expressed genes (DEGs) and proteins (DEPs) indicated that the inhibition of C. gloeosporioides by B. subtilis CF-3 VOCs downregulated the expression of genes related to cell membrane fluidity, cell wall integrity, energy metabolism, and production of cell wall-degrading enzymes. Particularly, those important DEGs and DEPs related to the ergosterol biosynthetic and biosynthesis of unsaturated fatty acids are most significantly influenced. 2,4-di-tert-butylphenol, a characteristic component of B. subtilis CF-3 VOCs, also showed a similar effect on C. gloeosporioides. Our results provide a theoretical basis for the potential application of B. subtilis CF-3 in the postharvest protection of fruits and vegetables" |
| Keywords: | Antifungal Agents Bacillus subtilis/genetics *Colletotrichum Plant Diseases Technology *Volatile Organic Compounds Bacillus subtilis anthracnose proteomics transcriptomics volatile organic compounds; |
| Notes: | "MedlineWang, Ke Qin, Zhen Wu, Shiyuan Zhao, Pengyu Zhen, Chaoying Gao, Haiyan eng 2021/04/27 J Agric Food Chem. 2021 May 5; 69(17):5267-5278. doi: 10.1021/acs.jafc.1c00640. Epub 2021 Apr 26" |
