| Title: | Evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ |
| Author(s): | Wang GY; Wang HH; Han YW; Xing T; Ye KP; Xu XL; Zhou GH; |
| Address: | "Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Collaborative Innovation Center of Food Safety and Nutrition, Ministry of Education and Finance, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: xlxus@njau.edu.cn" |
| ISSN/ISBN: | 1095-9998 (Electronic) 0740-0020 (Linking) |
| Abstract: | "Microorganisms play an important role in the spoilage of chilled chicken. In this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. Four isolates, namely Aeromonas salmonicida 35, Pseudomonas fluorescens H5, Pseudomonas fragi H8 and Serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage potential in situ. The in vitro studies showed that A. salmonicida 35 displayed the strongest proteolytic activity against both sarcoplasmic and myofibrillar proteins. However, the major spoilage isolate in situ was P. fragi H8, which exhibited a fast growth rate, slime formation and increased pH and total volatile basic nitrogen (TVBN) on chicken breast fillets. The relative amounts of volatile organic compounds (VOCs) originating from the microorganisms, including alcohols, aldehydes, ketones and several sulfur compounds, increased during storage. In sum, this study demonstrated the characteristics of 4 potential spoilage bacteria on chilled yellow-feather chicken and provides a simple and convenient method to assess spoilage bacteria during quality management" |
| Keywords: | Aeromonas salmonicida/growth & development/isolation & purification/*metabolism Animals Chickens/*microbiology Food Microbiology Food Storage Poultry/*microbiology Proteolysis Pseudomonas/growth & development/*metabolism Pseudomonas fluorescens/growth & d; |
| Notes: | "MedlineWang, Guang-Yu Wang, Hu-Hu Han, Yi-Wei Xing, Tong Ye, Ke-Ping Xu, Xing-Lian Zhou, Guang-Hong eng Evaluation Study England 2017/01/04 Food Microbiol. 2017 May; 63:139-146. doi: 10.1016/j.fm.2016.11.015. Epub 2016 Nov 24" |
