| Title: | Courtship Conditioning/Suppression Assays in Drosophila |
| Author(s): | von Philipsborn AC; Shohat-Ophir G; Rezaval C; |
| Address: | "Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic-EMBL Partnership for Molecular Medicine, Aarhus University, 8000 Aarhus, Denmark. The Mina and Everard Goodman Faculty of Life Sciences, The Leslie and Susan Gonda Multidisciplinary Brain Research Center, the Nanotechnology Institute, Bar-Ilan University, Ramat Gan 5290002, Israel galit.ophir@biu.ac.il c.rezaval@bham.ac.uk. School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom galit.ophir@biu.ac.il c.rezaval@bham.ac.uk" |
| ISSN/ISBN: | 1559-6095 (Electronic) 1559-6095 (Linking) |
| Abstract: | "Naive males court both virgin and mated females but learn through experience to discriminate between them, thus minimizing futile investments in nonreceptive female flies. In the laboratory, we can exploit the innate courtship enthusiasm of males and manipulate their behavior by placing them with a nonreceptive female (immature virgin females, decapitated mature virgin females, or mature mated females), termed as the courtship suppression/conditioning assay. Early studies showed that male flies that experience failure to mate upon interaction with nonreceptive previously mated females show decreased motivation to court (courtship suppression). Courtship suppression is an important experimental paradigm for studying genes and neuronal circuits that mediate short- and long-term memory. The anti-aphrodisiac male-specific pheromone 11-cis-vaccenyl-acetate plays a key role in this conditioned response, as male flies learn to associate its presence on mated females with the failure to mate" |
| Keywords: | "Animals Male Female *Drosophila *Drosophila melanogaster/genetics Courtship Sexual Behavior, Animal/physiology;neuroscience;" |
| Notes: | "Medlinevon Philipsborn, Anne C Shohat-Ophir, Galit Rezaval, Carolina eng WT_/Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't 2023/02/14 Cold Spring Harb Protoc. 2023 Jul 5; 2023(7):.pdb.prot108106. doi: 10.1101/pdb.prot108106" |
