| Title: | Characterisation of Acetyl-CoA Thiolase: The First Enzyme in the Biosynthesis of Terpenic Sex Pheromone Components in the Labial Gland of Bombus terrestris |
| Author(s): | Brabcova J; Demianova Z; Kindl J; Pichova I; Valterova I; Zarevucka M; |
| Address: | "Institute of Organic Chemistry and Biochemistry AS CR, 166 10 Prague 6 (Czech Republic)" |
| ISSN/ISBN: | 1439-7633 (Electronic) 1439-4227 (Linking) |
| Abstract: | "Buff-tailed bumblebees, Bombus terrestris, use a male sex pheromone for premating communication. Its main component is a sesquiterpene, 2,3-dihydrofarnesol. This paper reports the isolation of a thiolase (acetyl-CoA thiolase, AACT_BT), the first enzyme involved in the biosynthetic pathway leading to formation of isoprenoids in the B. terrestris male sex pheromone. Characterisation of AACT_BT might contribute to a better understanding of pheromonogenesis in the labial gland of B. terrestris males. The protein was purified to apparent homogeneity by column chromatography with subsequent stepwise treatment. AACT_BT showed optimum acetyltransferase activity at pH 7.1 and was strongly inhibited by iodoacetamide. The enzyme migrated as a band with an apparent mass of 42.9 kDa on SDS-PAGE. MS analysis of an AACT_BT tryptic digest revealed high homology to representatives of the thiolase family. AACT_BT has 96 % amino acid sequence identity with the previously reported Bombus impatiens thiolase" |
| Keywords: | "Acetyl-CoA C-Acetyltransferase/antagonists & inhibitors/chemistry/*metabolism Amino Acid Sequence Animals Bees/*metabolism Enzyme Inhibitors/pharmacology Hydrogen-Ion Concentration Male Molecular Sequence Data Molecular Weight Salivary Glands, Minor/*enzy;" |
| Notes: | "MedlineBrabcova, Jana Demianova, Zuzana Kindl, Jiri Pichova, Iva Valterova, Irena Zarevucka, Marie eng Research Support, Non-U.S. Gov't Germany 2015/03/25 Chembiochem. 2015 May 4; 16(7):1047-51. doi: 10.1002/cbic.201402591. Epub 2015 Mar 20" |
