| Title: | Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS) |
| Author(s): | Vallarino JG; Erban A; Fehrle I; Fernie AR; Kopka J; Osorio S; |
| Address: | "Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany. Departamento de Biologia Molecular y Bioquimica, Instituto de Hortofruticultura Subtropical y Mediterranea (IHSM), Universidad de Malaga-Consejo Superior de Investigaciones Cientificas, Malaga, Spain. sosorio@uma.es" |
| DOI: | 10.1007/978-1-4939-7819-9_16 |
| ISSN/ISBN: | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Abstract: | "Plants synthesize and emit a large range of volatile organic compounds (VOCs) that play important roles in their interactions with the environment, from attracting pollinators and seed dispersers to protectants such as repellants and pathogen inhibitors. As such, the development of techniques for headspace collection of volatiles in combination with gas chromatography-mass spectrometry (GC-MS) has an important impact on our understanding of the biosynthesis of plant VOCs. Furthermore, knowledge of the plant VOCs can be valuable in relation to plant breeding for improving fruit flavor or enhancing resistance to insects or pathogens. This chapter describes a reliable method for extracting volatile compounds by headspace solid-phase microextraction (HS-SPME), and separate and detect them by GC-MS" |
| Keywords: | Gas Chromatography-Mass Spectrometry/*methods Solid-Phase Synthesis Techniques Volatile Organic Compounds/*analysis Gas chromatography Hs-spme Headspace solid-phase microextraction Mass spectrometry Plant VOCs Volatile compounds; |
| Notes: | "MedlineVallarino, Jose G Erban, Alexander Fehrle, Ines Fernie, Alisdair R Kopka, Joachim Osorio, Sonia eng Research Support, Non-U.S. Gov't 2018/05/16 Methods Mol Biol. 2018; 1778:225-239. doi: 10.1007/978-1-4939-7819-9_16" |
