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Int J Mol Sci

Title:		An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
Author(s):		Ullah RMK; Quershi SR; Adeel MM; Abdelnabby H; Waris MI; Duan SG; Wang MQ;
Address:		"Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Yibin University, Yibin 644000, China. College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. Department of Plant Protection, Faculty of Agriculture, Benha University, Banha, Qalyubia 13736, Egypt"
Journal Title:		Int J Mol Sci
Year:		2020
Volume:		20201106
Issue:		21
Page Number:		-
DOI:		10.3390/ijms21218331
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, and behavioral tests were performed. Fluorescence binding assay results showed that SaveOBP9 had broad and high (Ki < 10 muM) binding abilities with most of the wheat volatiles, but was more obvious at pH 7.4 than pH 5.0. The binding sites of SaveOBP9 to the volatiles were predicted well by three-dimensional docking structure modeling and molecular docking. Moreover, S. avenae showed a strong behavioral response with the four compounds of wheat. The reduction in mRNA transcript levels after the RNA interference significantly reduced the expression level of SaveOBP9 and induced the non-significant response of S. avenae to the tetradecane, octanal, decanal, and hexadecane. This study provides evidence that SaveOBP9 might be involved in the chemoreception of wheat volatile organic compounds and can successfully contribute in the integrated management programs of S. avenae"
Keywords:		"Amino Acid Sequence/genetics Animals Aphids/genetics/*metabolism Carrier Proteins/metabolism Chemoreceptor Cells/metabolism Feeding Behavior Molecular Docking Simulation Odorants Plant Leaves/metabolism Receptors, Odorant/genetics/*metabolism/physiology S;"
Notes:		"MedlineUllah, Rana Muhammad Kaleem Quershi, Sundas Rana Adeel, Muhammad Muzammal Abdelnabby, Hazem Waris, Muhammad Irfan Duan, Shuang-Gang Wang, Man-Qun eng 2017YFD0200400/Ministry of Science and Technology of the People's Republic of China/ Switzerland 2020/11/12 Int J Mol Sci. 2020 Nov 6; 21(21):8331. doi: 10.3390/ijms21218331"