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Yeast

Title:		"The KlSTE2 and KlSTE3 genes encode MATalpha- and MATa-specific G-protein-coupled receptors, respectively, which are required for mating of Kluyveromyces lactis haploid cells"
Author(s):		Torres-Quiroz F; Kawasaki L; Rodriguez-Gonzalez M; Patron-Soberano A; Coria R;
Address:		"Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Apartado Postal 70-242, 04510 Mexico, D.F., Mexico"
Journal Title:		Yeast
Year:		2007
Volume:		24
Issue:		1
Page Number:		17 - 25
DOI:		10.1002/yea.1432
ISSN/ISBN:		0749-503X (Print) 0749-503X (Linking)
Abstract:		"Mating in yeast is initiated by binding of pheromone to G-protein-coupled receptors expressed in haploid cells. We analysed the role of KlSte2p and KlSte3p receptors in the Kluyveromyces lactis mating pathway. By sequence analysis, KlSte2p and KlSte3p are the homologues of the Saccharomyces cerevisiae alpha-pheromone and a-pheromone receptors, respectively. However, by expression experiments, we determined that KlSTE2 gene is expressed in the cells typified as MATalpha and therefore is the receptor for the K. lactis a-pheromone and KlSTE3 gene is expressed in the MATa cells and binds the alpha-pheromone. The KlSTE2 gene is silent in MATa cells, while it is highly expressed in MATalpha cells, and conversely the KlSTE3 gene is expressed in MATa cells and repressed in MATalpha cells. Disruption mutants of both genes were found to be sterile, and this defect is reversed by plasmidic copies of each gene. The cytoplasmic C-terminus of KlSte3p interacts strongly with the KlGpa1p (Galpha) subunit, which is involved in the transduction of the pheromone stimulus to induce mating. Remarkably, this same domain does not interact with a constitutive active allele of the Galpha subunit, indicating that the C-terminus is able to discriminate between the active (GTP-bound) and inactive (GDP-bound) forms of the Galpha subunit"
Keywords:		"Amino Acid Sequence Blotting, Northern DNA, Fungal/chemistry/genetics Gene Expression Regulation, Fungal Genes, Mating Type, Fungal/genetics/*physiology Haploidy Kluyveromyces/genetics/*physiology Microscopy, Confocal Molecular Sequence Data Mutagenesis, ;"
Notes:		"MedlineTorres-Quiroz, Francisco Kawasaki, Laura Rodriguez-Gonzalez, Miriam Patron-Soberano, Araceli Coria, Roberto eng Research Support, Non-U.S. Gov't England 2006/12/29 Yeast. 2007 Jan; 24(1):17-25. doi: 10.1002/yea.1432"