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J Microbiol Methods

Title:		Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro
Author(s):		Thorn RM; Reynolds DM; Greenman J;
Address:		"Centre for Research in Biomedicine, Department of Applied Sciences, University of the West of England, Bristol, Frenchay Campus, United Kingdom. Robin2.Thorn@uwe.ac.uk"
Journal Title:		J Microbiol Methods
Year:		2011
Volume:		20101216
Issue:		2
Page Number:		258 - 264
DOI:		10.1016/j.mimet.2010.12.001
ISSN/ISBN:		1872-8359 (Electronic) 0167-7012 (Linking)
Abstract:		"Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro. Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was used to quantify the concentration of 23 microbial VCs within the head-space of various bacterial monocultures, during both the logarithmic and stationary growth phases. In comparison with existing techniques, SIFT-MS enables quantitative, high throughput, real-time head-space analysis to be performed, without need for sample preparation. The results show that most VCs were produced by >1 bacterial species or strain, and some were produced by all strains tested. Multivariate analysis using similarity matrices, cluster analysis and multidimensional scaling (MDS) was used to determine whether there was a characteristic VC profile at either the species or strain level. Significant discrimination of all bacterial species and strains was achieved by analysing the VC profiles, and the relative similarity of VC profiles could be differentiated in 2 or 3 dimensional space. This study has shown that there are significant differences in the volatile profiles obtained from various bacterial monocultures grown in vitro, and that the analysis techniques herein employed have the potential to differentiate samples at the strain level"
Keywords:		Bacteria/*chemistry/*classification/metabolism Bacteriological Techniques/*methods Cluster Analysis Mass Spectrometry/*methods *Metabolome Multivariate Analysis Sensitivity and Specificity Volatile Organic Compounds/*analysis;
Notes:		"MedlineThorn, Robin Michael Statham Reynolds, Darren Michael Greenman, John eng Evaluation Study Netherlands 2010/12/21 J Microbiol Methods. 2011 Feb; 84(2):258-64. doi: 10.1016/j.mimet.2010.12.001. Epub 2010 Dec 16"