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Trop Biomed

Title:		Expression of cytosolic and thiolated proteome of Musca domestica larvae under oxidative challenge
Author(s):		Tan YH; Alias Z;
Address:		"Toxicology and Protein Analysis Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia"
Journal Title:		Trop Biomed
Year:		2020
Volume:		37
Issue:		3
Page Number:		744 - 755
DOI:		10.47665/tb.37.3.744
ISSN/ISBN:		2521-9855 (Electronic) 0127-5720 (Linking)
Abstract:		"The study was aimed to investigate the expression of cytosolic and thiolated proteins of Musca domestica larvae under oxidative stress. Proteins from acute treatment of hydrogen peroxide (LC(50) = 21.52% (v/v)) on 3rd stage larvae of housefly were extracted and purified using an activated Thiol Sepharose(R) for thiolated protein purification. Two dimensional gel electrophoresis was used for visualizing and analyzing expression of cytosolic and thiolated proteins. Protein spots with more than 5 fold of expression change were identified using liquid chromatography- tandem mass spectrometry (LC-MS/MS). The cytosolic proteins were actin, tropomyosin, ubiquitin, arginine kinase, pheromone binding protein/general odorant binding protein, and ATP: guanidino phosphotransferase. The thiolated proteins with more than 5 fold change in expression as an effect to the acute treatment were fructose bisphosphate aldolase, short chain dehydrogenase and lactate/malate dehydrogenase. The proteins identified in the study should provide vital information for future reference in oxidative stress defence and response occurring in houseflies"
Keywords:		Animals Cytosol/*metabolism Houseflies/*metabolism Larva Lipid Peroxidation *Oxidative Stress *Proteome;
Notes:		"MedlineTan, Y H Alias, Z eng Malaysia 2021/02/23 Trop Biomed. 2020 Sep 1; 37(3):744-755. doi: 10.47665/tb.37.3.744"