| Title: | "Combinatorial multispectral, thermodynamics, docking and site-directed mutagenesis reveal the cognitive characteristics of honey bee chemosensory protein to plant semiochemical" |
| Author(s): | Tan J; Song X; Fu X; Wu F; Hu F; Li H; |
| Address: | "Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China. Electronic address: hlli@cjlu.edu.cn" |
| Journal Title: | Spectrochim Acta A Mol Biomol Spectrosc |
| DOI: | 10.1016/j.saa.2018.04.074 |
| ISSN/ISBN: | 1873-3557 (Electronic) 1386-1425 (Linking) |
| Abstract: | "In the chemoreceptive system of insects, there are always some soluble binding proteins, such as some antennal-specific chemosensory proteins (CSPs), which are abundantly distributed in the chemosensory sensillar lymph. The antennal-specific CSPs usually have strong capability to bind diverse semiochemicals, while the detailed interaction between CSPs and the semiochemicals remain unclear. Here, by means of the combinatorial multispectral, thermodynamics, docking and site-directed mutagenesis, we detailedly interpreted a binding interaction between a plant semiochemical beta-ionone and antennal-specific CSP1 from the worker honey bee. Thermodynamic parameters (DeltaH?ª+?ª+0) indicate that the interaction is mainly driven by hydrophobic forces and electrostatic interactions. Docking prediction results showed that there are two key amino acids, Phe44 and Gln63, may be involved in the interacting process of CSP1 to beta-ionone. In order to confirm the two key amino acids, site-directed mutagenesis were performed and the binding constant (K(A)) for two CSP1 mutant proteins was reduced by 60.82% and 46.80% compared to wild-type CSP1. The thermodynamic analysis of mutant proteins furtherly verified that Phe44 maintained an electrostatic interaction and Gln63 contributes hydrophobic and electrostatic forces. Our investigation initially elucidates the physicochemical mechanism of the interaction between antennal-special CSPs in insects including bees to plant semiochemicals, as well as the development of twice thermodynamic analysis (wild type and mutant proteins) combined with multispectral and site-directed mutagenesis methods" |
| Keywords: | "Animals Bees/*chemistry Insect Proteins/*chemistry/genetics/*metabolism Molecular Docking Simulation Mutagenesis, Site-Directed Norisoprenoids/chemistry/metabolism Pheromones/chemistry/metabolism Phytochemicals/*chemistry/*metabolism Protein Binding Spect;" |
| Notes: | "MedlineTan, Jing Song, Xinmi Fu, Xiaobin Wu, Fan Hu, Fuliang Li, Hongliang eng England 2018/05/16 Spectrochim Acta A Mol Biomol Spectrosc. 2018 Aug 5; 201:346-353. doi: 10.1016/j.saa.2018.04.074. Epub 2018 May 9" |
