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Chem Res Toxicol

Title:		Development of an Integrated Platform to Assess the Physicochemical and Toxicological Properties of Wood Combustion Particulate Matter
Author(s):		Singh D; Tassew DD; Nelson J; Chalbot MG; Kavouras IG; Demokritou P; Tesfaigzi Y;
Address:		"Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, 665 Huntington Avenue, Boston, Massachusetts 02115, United States. Environmental and Occupational Health Sciences Institute, School of Public Health, Rutgers University, 170 Frelinghuysen Road, Piscataway, New Jersey 08854, United States. Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, Massachusetts 02115, United States. Department of Environmental Health Sciences, University of Alabama at Birmingham, 1600 University Blvd, Birmingham, Alabama 35216, United States. Department of Environmental, Occupational, and Geospatial Health Sciences, CUNY Graduate School of Public Health & Health Policy, 55 West 125th Street, New York, New York 10027, United States"
Journal Title:		Chem Res Toxicol
Year:		2022
Volume:		20220906
Issue:		9
Page Number:		1541 - 1557
DOI:		10.1021/acs.chemrestox.2c00183
ISSN/ISBN:		1520-5010 (Electronic) 0893-228X (Print) 0893-228X (Linking)
Abstract:		"Wood burning contributes to indoor and ambient particulate matter (PM) pollution and has been associated with increased morbidity and mortality. Here, we present an integrated methodology that allows to generate, sample, and characterize wood smoke derived from different moisture contents and representative combustion conditions using pine wood as a model. Flaming, smoldering, and incomplete combustion were assessed for low-moisture pine, whereas both low-moisture pine and high-moisture pine were investigated under flaming conditions. Real-time monitoring of carbon monoxide, volatile organic compounds, and aerosol number concentration/size in wood smoke was performed. The PM was size-fractionated, sampled, and characterized for elemental/organic carbon, organic functional groups, and inorganic elements. Bioactivity of PM was assessed by measuring the sterile alpha motif (SAM) pointed domain containing ETS (E-twenty-six) transcription factor (SPDEF) gene promoter activity in human embryonic kidney 293 (HEK-293T) cells, a biomarker for mucin gene expression. Findings showed that moisture content and combustion condition significantly affected the organic and inorganic elemental composition of PM(0.1) as well as its bioactivity. Also, for a given moisture and combustion scenario, PM chemistry and bioactivity differed considerably with PM size. Importantly, PM(0.1) from flaming combustion of low-moisture pine contained the highest abundance of the oxygenated saturated aliphatic functional group [H-C-O] and was also biologically most potent in stimulating SPDEF promoter activity, suggesting the role of organic compounds such as carbohydrates and sugar alcohols (that contain [H-C-O]) in driving mucus-related respiratory outcomes. Our platform enables further well-controlled parametric studies using a combination of in vitro and in vivo approaches to link wood burning parameters with acute and chronic inhalation health effects of wood smoke"
Keywords:		*Air Pollutants/analysis/toxicity Carbohydrates/analysis Carbon Monoxide/analysis/toxicity Humans Mucins/analysis *Particulate Matter/analysis/toxicity *Smoke/adverse effects/analysis Sugar Alcohols/analysis Transcription Factors *Volatile Organic Compoun;
Notes:		"MedlineSingh, Dilpreet Tassew, Dereje Damte Nelson, Jordan Chalbot, Marie-Cecile G Kavouras, Ilias G Demokritou, Philip Tesfaigzi, Yohannes eng P30 ES005022/ES/NIEHS NIH HHS/ R01 HL068111/HL/NHLBI NIH HHS/ R01 HL140839/HL/NHLBI NIH HHS/ U24 ES026946/ES/NIEHS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2022/09/07 Chem Res Toxicol. 2022 Sep 19; 35(9):1541-1557. doi: 10.1021/acs.chemrestox.2c00183. Epub 2022 Sep 6"