| Title: | Biosensor development for low-level acetaldehyde gas detection using mesoporous carbon electrode printed on a porous polyimide film |
| Author(s): | Shitanda I; Oshimoto T; Loew N; Motosuke M; Watanabe H; Mikawa T; Itagaki M; |
| Address: | "Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan. Electronic address: shitanda@rs.tus.ac.jp. Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan. Department of Mechanical Engineering, Faculty of Engineering, Tokyo University of Science, 3-1, Shinjuku 6-chome, Katsushika-ku, Tokyo, 125-8585, Japan. RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan" |
| DOI: | 10.1016/j.bios.2023.115555 |
| ISSN/ISBN: | 1873-4235 (Electronic) 0956-5663 (Linking) |
| Abstract: | "Acetaldehyde, which is an intermediate product of alcohol metabolism, is known to induce symptoms, including alcohol flushing, vomiting, and headaches in humans. Therefore, real-time monitoring of acetaldehyde levels is crucial to mitigating these health issues. However, current methods for detecting low-concentration gases necessitate the use of complex measurement equipment. In this study, we developed a low-cost, low-detection-limit, enzyme-based electrochemical biosensor for acetaldehyde gas detection that does not require sophisticated equipment. The sensor was constructed by screen-printing electrodes onto a porous polyimide film, using grafted MgO-templated carbon (GMgOC) as working electrode material, carbon for the counter electrode, and silver/silver chloride for the reference electrode. Pyrroloquinoline-quinone-dependent aldehyde dehydrogenase was immobilized on the working electrode, and a chamber was attached to the electrode chip and filled with 1-methoxy-5-methylphenazinium methyl sulfate solution. The sensor can be used to measure acetaldehyde gas concentrations from 0.02 to 0.1 ppm, making it suitable for monitoring human skin gas. This low detection limit was achieved by delivering the analyte through the porous polyimide film on which the electrodes were printed and accumulating acetaldehyde in the mesoporous GMgOC of the working electrode. This mechanism suggests that this sensor design can be adapted to develop other low-detection limit gas sensors, such as those for screening skin gas biomarkers" |
| Keywords: | Humans *Carbon *Biosensing Techniques/methods Acetaldehyde Porosity Electrodes Acetaldehyde detection Enzyme sensor Mesoporous electrode Skin gas sensor Volatile organic compound; |
| Notes: | "MedlineShitanda, Isao Oshimoto, Taisei Loew, Noya Motosuke, Masahiro Watanabe, Hikari Mikawa, Tsutomu Itagaki, Masayuki eng England 2023/08/06 Biosens Bioelectron. 2023 Oct 15; 238:115555. doi: 10.1016/j.bios.2023.115555. Epub 2023 Jul 26" |
