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J Gen Appl Microbiol

Title:		Inhibitory effect of Lactococcus lactis on the bioactivity of periodontopathogens
Author(s):		Shin HS; Baek DH; Lee SH;
Address:		"Department of Periodontology, College of Dentistry, Dankook University. Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University"
Journal Title:		J Gen Appl Microbiol
Year:		2018
Volume:		20180125
Issue:		2
Page Number:		55 - 61
DOI:		10.2323/jgam.2017.06.003
ISSN/ISBN:		1349-8037 (Electronic) 0022-1260 (Linking)
Abstract:		"Lactococcus lactis is a probiotic bacterium that produces various bacteriocins. Periodontopathogens induce inflammation and halitosis through the actions of lipopolysaccharide (LPS) and trypsin-like enzymes. The purpose of this study was to investigate the inhibitory effects of L. lactis on the bioactivity of periodontopathogens. To investigate the antimicrobial peptide of L. lactis, the spent culture medium (SCM) of L. lactis was treated with or without proteinase K after collection by centrifugation, and the antibacterial activity of SCM against periodontopathogens was assessed. To evaluate the neutralizing effect of L. lactis on halitosis, SCM of periodontopathogens was mixed with an L. lactis suspension, and the levels of volatile sulfur compounds (VSCs) were measured by gas chromatography. LPS from the periodontopathogens was extracted by an LPS extraction kit with little modification, and THP-1 cells as a monocytic cell line were treated with the extracted LPS in the presence or absence of UV-killed L. lactis. The production of inflammatory cytokines was analyzed by ELISA. The SCM of L. lactis exhibited antimicrobial activity against the periodontopathogens, whereas the proteinase K-treated SCM showed little antimicrobial activity. In addition, the L. lactis suspension had a neutralizing effect on the VSCs produced by periodontopathogens, and UV-killed L. lactis inhibited the production of IL-6 and TNF-alpha induced by the LPS. These results suggest that L. lactis may be a useful probiotic to prevent and treat periodontitis and halitosis"
Keywords:		"Antibiosis/*physiology Bacteria/chemistry/growth & development/metabolism *Bacterial Physiological Phenomena Culture Media, Conditioned Cytokines/metabolism Humans Lactococcus lactis/metabolism/*physiology Lipopolysaccharides/physiology Periodontitis/*mic;"
Notes:		"MedlineShin, Hyun-Seung Baek, Dong-Heon Lee, Sung-Hoon eng Japan 2018/01/26 J Gen Appl Microbiol. 2018 May 21; 64(2):55-61. doi: 10.2323/jgam.2017.06.003. Epub 2018 Jan 25"