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Yeast

Title:		Isolation of a gene encoding a G protein alpha subunit involved in the regulation of cAMP levels in the yeast Kluyveromyces lactis
Author(s):		Savinon-Tejeda AL; Ongay-Larios L; Ramirez J; Coria R;
Address:		"Departamento de Microbiologia, Universidad Nacional Autonoma de Mexico, Mexico, D.F., Mexico"
Journal Title:		Yeast
Year:		1996
Volume:		12
Issue:		11
Page Number:		1125 - 1133
DOI:		10.1002/(SICI)1097-0061(19960915)12:11%3C1125::AID-YEA7%3E3.0.CO;2-2
ISSN/ISBN:		0749-503X (Print) 0749-503X (Linking)
Abstract:		"Using chromosomal DNA from Kluyveromyces lactis as template and oligodeoxynucleotides designed from conserved regions of various G protein alpha subunits we were able to amplify by the polymerase chain reaction two products of approximately 0.5 kb (P-1) and 0.8 kb (P-2). Sequencing showed that these two fragments share high homology with genes coding for the G alpha subunits from different sources. Using the P-1 fragment as a probe we screened a genomic library from K. lactis and we cloned a gene (KlGPA2) whose deduced amino acid sequence showed, depending on the exact alignment, 62% similarity and 38% identity with Gpa1p and 76% similarity and 63% identity with Gpa2p, the G protein alpha subunits from Saccharomyces cerevisiae. KlGPA2 is a single-copy gene and its disruption rendered viable cells with significantly reduced cAMP level, indicating that this G alpha subunit may be involved in regulating the adenylyl cyclase activity, rather than participating in the mating pheromone response pathway. KlGpa2p shares some structural similarities with members of the mammalian G alpha s family (stimulatory of adenylyl cyclase) including the absence in its N-terminus of a myristoyl-modification sequence"
Keywords:		"Amino Acid Sequence Base Sequence Cyclic AMP/*biosynthesis GTP-Binding Proteins/*genetics/physiology *Genes, Fungal Kluyveromyces/*genetics Molecular Sequence Data;"
Notes:		"MedlineSavinon-Tejeda, A L Ongay-Larios, L Ramirez, J Coria, R eng Research Support, Non-U.S. Gov't England 1996/09/15 Yeast. 1996 Sep 15; 12(11):1125-33. doi: 10.1002/(SICI)1097-0061(19960915)12:11%3C1125::AID-YEA7%3E3.0.CO; 2-2"