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Biosens Bioelectron

Title:		Odorant binding protein based biomimetic sensors for detection of alcohols associated with Salmonella contamination in packaged beef
Author(s):		Sankaran S; Panigrahi S; Mallik S;
Address:		"Agricultural and Biosystems Engineering, North Dakota State University, Fargo, ND 58108, USA"
Journal Title:		Biosens Bioelectron
Year:		2011
Volume:		20100827
Issue:		7
Page Number:		3103 - 3109
DOI:		10.1016/j.bios.2010.07.122
ISSN/ISBN:		1873-4235 (Electronic) 0956-5663 (Linking)
Abstract:		"Detection of food-borne bacteria present in the food products is critical to prevent the spread of infectious diseases. Intelligent quality sensors are being developed for detecting bacterial pathogens such as Salmonella in beef. One of our research thrusts was to develop novel sensing materials sensitive to specific indicator alcohols at low concentrations. Present work focuses on developing olfactory sensors mimicking insect odorant binding protein to detect alcohols in low concentrations at room temperature. A quartz crystal microbalance (QCM) based sensor in conjunction with synthetic peptide was developed to detect volatile organic compounds indicative to Salmonella contamination in packaged beef. The peptide sequence used as sensing materials was derived from the amino acids sequence of Drosophila odorant binding protein, LUSH. The sensors were used to detect alcohols: 3-methyl-1-butanol and 1-hexanol. The sensors were sensitive to alcohols with estimated lower detection limits of <5 ppm. Thus, the LUSH-derived QCM sensors exhibited potential to detect alcohols at low ppm concentrations"
Keywords:		Amino Acid Sequence Animals Biomimetic Materials/metabolism Biosensing Techniques/*instrumentation/methods Cattle Drosophila/metabolism Drosophila Proteins/*metabolism Equipment Design Hexanols/*analysis/metabolism Limit of Detection Meat Products/microbi;
Notes:		"MedlineSankaran, Sindhuja Panigrahi, Suranjan Mallik, Sanku eng Evaluation Study Research Support, U.S. Gov't, Non-P.H.S. England 2011/01/14 Biosens Bioelectron. 2011 Mar 15; 26(7):3103-9. doi: 10.1016/j.bios.2010.07.122. Epub 2010 Aug 27"