| Title: | CsTPS21 encodes a jasmonate-responsive monoterpene synthase producing beta-ocimene in citrus against Asian citrus psyllid |
| Author(s): | Bin M; Peng X; Yi G; Zhang X; |
| Address: | "Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou, 510640, China; College of Life Sciences, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China. Electronic address: binminliang@163.com. College of Life Sciences, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China. Electronic address: xpeng@scau.edu.cn. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou, 510640, China. Electronic address: yiganjun@vip.163.com. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou, 510640, China. Electronic address: zhangxinxin@gdaas.cn" |
| DOI: | 10.1016/j.plaphy.2023.107887 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "Huanglongbing (HLB), spread by the Asian citrus psyllid (ACP), is a widespread, devastating disease that causes significant losses in citrus production. Therefore, controlling the ACP infestation and HLB infection is very important for citrus production. The aim of our study was to identify any citrus volatile which could be used as a repellent or less attractant towards ACP, and to envisage the potential of this strategy to control HLB spread. The present study identified a terpene synthase (TPS)-encoding gene CsTPS21 in citrus plants, and this gene was predicted to encode a monoterpene synthase and had an amino acid sequence similar to beta-ocimene synthase. CsTPS21 was significantly upregulated by ACP infestation and methyl jasmonic acid (MeJA) treatment but downregulated by salicylic acid (SA). Further heterologous gene expression studies in yeast cells and tobacco plants indicated that the protein catalyzed the formation of beta-ocimene, which acted as an ACP repellent. Detailed analysis of tobacco overexpressing CsTPS21 showed that CsTPS21 synthesizing beta-ocimene regulated jasmonic acid (JA)-associated pathways by increasing the JA accumulation and inducing the JA biosynthetic gene expression to defend against insect infestation. These findings provide a basis to plan strategies to manage HLB in the field using beta-ocimene and CsTPS21 as candidates" |
| Keywords: | Animals *Citrus/genetics/metabolism *Hemiptera Plant Diseases/genetics Asian citrus psyllid Citrus sinensis CsTPS21 Herbivore defense JA pathway beta-ocimene; |
| Notes: | "MedlineBin, Minliang Peng, Xinxiang Yi, Ganjun Zhang, Xinxin eng France 2023/07/14 Plant Physiol Biochem. 2023 Aug; 201:107887. doi: 10.1016/j.plaphy.2023.107887. Epub 2023 Jul 10" |
