| Title: | Nutrigenomic analyses reveal miRNAs and mRNAs affected by feed restriction in the mammary gland of midlactation dairy cows |
| Author(s): | Billa PA; Faulconnier Y; Ye T; Bourdon C; Pires JAA; Leroux C; |
| Address: | "INRAE, Universite Clermont Auvergne, VetAgro Sup, UMR Herbivores, Saint-Genes-Champanelle, France. Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique, UMR7104, Institut National de la Sante et de la Recherche Medicale, U964, Universite de Strasbourg, Illkirch, France. INRAE, AgroParisTech, Universite Paris-Saclay, UMR Genetique Animale et Biologie Integrative, Jouy-en-Josas, France" |
| DOI: | 10.1371/journal.pone.0248680 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "The objective of this study was to investigate the effects of feed restriction on mammary miRNAs and coding gene expression in midlactation cows. Five Holstein cows and 6 Montbeliarde cows underwent 6 days of feed restriction, during which feed allowance was reduced to meet 50% of their net energy for lactation requirements. Mammary biopsies were performed before and at the end of the restriction period. Mammary miRNA and mRNA analyses were performed using high-throughput sequencing and microarray analyses, respectively. Feed restriction induced a negative energy balance and decreased milk production and fat and protein yields in both breeds. Feed restriction modified the expression of 27 miRNAs and 374 mRNAs in mammary glands from Holstein cows, whereas no significant miRNA change was observed in Montbeliarde cows. Among the 27 differentially expressed miRNAs, 8 miRNAs were associated with dairy QTL. Analysis of target genes indicate that the 8 most abundantly expressed miRNAs control transcripts related to lipid metabolism, mammary remodeling and stress response. A comparison between the mRNAs targeted by the 8 most strongly expressed miRNAs and 374 differentially expressed mRNAs identified 59 mRNAs in common. The bioinformatic analyses of these 59 mRNAs revealed their implication in lipid metabolism and endothelial cell proliferation. These effects of feed restriction on mammary miRNAs and mRNAs observed in Holstein cows suggest a potential role of miRNAs in mammary structure and lipid biosynthesis that could explain changes in milk production and composition" |
| Keywords: | "Animal Feed/*analysis Animals Cattle Cell Proliferation/genetics Energy Metabolism Female Food Deprivation/*physiology Gene Expression High-Throughput Nucleotide Sequencing Lactation/*genetics Lipid Metabolism/physiology Lipogenesis Mammary Glands, Animal;" |
| Notes: | "MedlineBilla, Pierre-Alexis Faulconnier, Yannick Ye, Tao Bourdon, Celine Pires, Jose A A Leroux, Christine eng Research Support, Non-U.S. Gov't 2021/04/16 PLoS One. 2021 Apr 15; 16(4):e0248680. doi: 10.1371/journal.pone.0248680. eCollection 2021" |
