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ISME J

Title:		Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta
Author(s):		Bilcke G; Van den Berge K; De Decker S; Bonneure E; Poulsen N; Bulankova P; Osuna-Cruz CM; Dickenson J; Sabbe K; Pohnert G; Vandepoele K; Mangelinckx S; Clement L; De Veylder L; Vyverman W;
Address:		"Protistology and Aquatic Ecology, Department of Biology, Ghent University, 9000, Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium. VIB Center for Plant Systems Biology, 9052, Ghent, Belgium. Department of Applied Mathematics, Computer Science and Statistics, Ghent University, 9000, Ghent, Belgium. Department of Statistics, University of California, Berkeley, Berkeley, CA, USA. SynBioC, Department of Green Chemistry and Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium. B CUBE Center for Molecular Bioengineering, Technical University of Dresden, Tatzberg 41, 01307, Dresden, Germany. Bioinformatics Institute Ghent, Ghent University, Technologiepark 71, 9052, Ghent, Belgium. Marine Biological Association, The Laboratory, Citadel Hill, Plymouth, PL1 2PB, UK. School of Ocean and Earth Science, University of Southampton, Southampton, UK. Bioorganic Analytics, Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, Lessingstr. 8, 07743, Jena, Germany. Protistology and Aquatic Ecology, Department of Biology, Ghent University, 9000, Ghent, Belgium. wim.vyverman@ugent.be"
Journal Title:		ISME J
Year:		2021
Volume:		20201007
Issue:		2
Page Number:		562 - 576
DOI:		10.1038/s41396-020-00797-7
ISSN/ISBN:		1751-7370 (Electronic) 1751-7362 (Print) 1751-7362 (Linking)
Abstract:		"Sexual reproduction is a fundamental phase in the life cycle of most diatoms. Despite its role as a source of genetic variation, it is rarely reported in natural circumstances and its molecular foundations remain largely unknown. Here, we integrate independent transcriptomic datasets to prioritize genes responding to sex inducing pheromones (SIPs) in the pennate diatom Seminavis robusta. We observe marked gene expression changes associated with SIP treatment in both mating types, including an inhibition of S phase progression, chloroplast division, mitosis, and cell wall formation. Meanwhile, meiotic genes are upregulated in response to SIP, including a sexually induced diatom specific cyclin. Our data further suggest an important role for reactive oxygen species, energy metabolism, and cGMP signaling during the early stages of sexual reproduction. In addition, we identify several genes with a mating type specific response to SIP, and link their expression pattern with physiological specialization, such as the production of the attraction pheromone diproline in mating type - (MT-) and mate-searching behavior in mating type + (MT+). Combined, our results provide a model for early sexual reproduction in pennate diatoms and significantly expand the suite of target genes to detect sexual reproduction events in natural diatom populations"
Keywords:		*Diatoms/genetics Pheromones Reproduction *Sex Attractants Transcriptome;
Notes:		"MedlineBilcke, Gust Van den Berge, Koen De Decker, Sam Bonneure, Eli Poulsen, Nicole Bulankova, Petra Osuna-Cruz, Cristina Maria Dickenson, Jack Sabbe, Koen Pohnert, Georg Vandepoele, Klaas Mangelinckx, Sven Clement, Lieven De Veylder, Lieven Vyverman, Wim eng Research Support, Non-U.S. Gov't England 2020/10/09 ISME J. 2021 Feb; 15(2):562-576. doi: 10.1038/s41396-020-00797-7. Epub 2020 Oct 7"