| Title: | "Effects of copper oxide nanoparticle on gill filtration rate, respiration rate, hemocyte associated immune parameters and oxidative status of an Indian freshwater mussel" |
| Author(s): | Ray A; Gautam A; Das S; Pal K; Das S; Karmakar P; Ray M; Ray S; |
| Address: | "Aquatic Toxicology Laboratory, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India. Nanobiotechnology Laboratory, Department of Life Science and Biotechnology, Jadavpur University, 188 Raja Subodh Chandra Mallick Road, Kolkata 700032, West Bengal, India. Semiochemicals and Lipid Laboratory, Department of Life Science, Presidency University, 86/1, College Street, Kolkata 700073, West Bengal, India. Aquatic Toxicology Laboratory, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India. Electronic address: raysnailmail@rediffmail.com" |
| Journal Title: | Comp Biochem Physiol C Toxicol Pharmacol |
| DOI: | 10.1016/j.cbpc.2020.108855 |
| ISSN/ISBN: | 1532-0456 (Print) 1532-0456 (Linking) |
| Abstract: | "Waterbodies of India support a wide range of molluscs including Lamellidens marginalis, a pearl forming edible mussel of ecological significance. Report of copper oxide nanoparticle toxicity in Indian molluscs is limited in scientific literature. L. marginalis is a gill respiring filter feeder, which is toxicologically vulnerable to exposure of copper oxide nanoparticles liberated from electrical, textile and polymer industries. Experimental exposure of copper oxide nanoparticles for 7 days yielded a decrease in gill filtration rate, respiration rate, total count and phagocytic response of hemocytes, the chief immunoeffector cells of L. marginalis. Nanoparticle exposure resulted in decrease of phagocytic response of mussel hemocytes. Decrease in nitric oxide generation and phenoloxidase activity were recorded in L. marginalis exposed to 0.5, 1 and 5 mg copper oxide nanoparticles per litre of water for 7 and 14 days. Superoxide anion generation in hemocytes was increased under the exposure of copper oxide nanoparticles. Increase in superoxide anion and decrease in the activities of superoxide dismutase and catalase were indicative to oxidative stress in mussels. Copper oxide nanoparticle induced shift in filtration and respiration rate along with the hemocyte associated immune parameters were suggestive to an acute immunophysiological stress in L. marginalis. We estimated the functional performance of gill and physiological status of aquatic respiration in L. marginalis exposed to copper oxide nanoparticles. A parallel set of estimation of each parameter was carried out in L. marginalis exposed to identical copper sulphate concentrations to record and compare the ionic toxicity of copper in the same specimen" |
| Keywords: | Animals Bivalvia/drug effects/*physiology Catalase/metabolism Copper/*toxicity Fresh Water Gills/drug effects/*physiology Hemocytes/*drug effects India Nanoparticles/*toxicity Nitric Oxide/metabolism Oxidative Stress/*drug effects Phagocytosis Respiratory; |
| Notes: | "MedlineRay, Abhishek Gautam, Arunodaya Das, Santanu Pal, Kunal Das, Subhadeep Karmakar, Parimal Ray, Mitali Ray, Sajal eng 2020/08/12 Comp Biochem Physiol C Toxicol Pharmacol. 2020 Nov; 237:108855. doi: 10.1016/j.cbpc.2020.108855. Epub 2020 Aug 8" |
