| Title: | Antimicrobial activity and chemical composition of the essential oil of Hofmeisteria schaffneri |
| Author(s): | Perez-Vasquez A; Capella S; Linares E; Bye R; Angeles-Lopez G; Mata R; |
| Address: | "Departamento de Farmacia, Facultad de Quimica Instituto de Biologia, Universidad Nacional Autonoma de Mexico, Mexico, Mexico" |
| DOI: | 10.1111/j.2042-7158.2010.01243.x |
| ISSN/ISBN: | 2042-7158 (Electronic) 0022-3573 (Linking) |
| Abstract: | "OBJECTIVES: The aims of this study were to establish the antimicrobial potential of Hofmeisteria schaffneri essential oil and its chemical composition. METHODS: The essential oils of Hofmeisteria schaffneri harvested at flowering (batches I and IV) and non-flowering (batches II and III) seasons were prepared by hydrodistillation and analysed by GC and GC-MS. The aqueous and organic (CH(2) Cl(2) -MeOH 1 : 1) extracts were prepared by using infusion and maceration techniques, respectively. The in-vitro antimicrobial activity of the preparations and compounds against Candida albicans and some bacteria (Gram-negative and Gram-positive) was assessed using the broth dilution method in 96-microplate wells. KEY FINDINGS: Forty-four compounds, representing approximately 90% of the total constituents, were identified in the essential oil of Hofmeisteria schaffneri collected in flowering (batches I and IV) and non-flowering (batches II and III) seasons. In all cases, several thymol analogues were the major components of the oils ( approximately 65%); some small differences in the relative proportions of these constituents were observed. The infusion exhibited an antibacterial activity against Staphylococcus aureus and Bacillus subtilis, with a MIC value of 64 microg/ml in each case. The essential oil batches were active against Staphylococcus aureus, with MIC ranging from 48 to 192 microg/ml. They were, however, inactive against Gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi (MIC > 1024 microg/ml). On the other hand, the infusion of the plant as well as the oil from batch I displayed anti-Candida albicans activity, with MIC of 128 and 192 microg/ml, respectively. Finally, the organic extract did not displayed significant activity against the tested microorganisms (MIC >/= 1024 microg/ml). Some of the compounds isolated from the plant were also tested. Compounds 8 and 38, which were present in the essential oils, displayed the best antibacterial effect against Gram-positive bacteria (MIC ranging between 32 and 64 microg/ml). Compounds 6 (present in the infusion) and 10 (present in all preparations) showed higher activity against the yeast (MIC = 128 microg/ml) than the remaining compounds, with MIC values ranging from 256 to 512 microg/ml. CONCLUSIONS: The composition and antimicrobial activity of the oils changed slightly from flowering to non-flowering seasons. The results of the present investigation provide in-vitro scientific support for the use of the plant against skin infections in Mexican folk medicine" |
| Keywords: | "Anti-Infective Agents/chemistry/isolation & purification/*pharmacology Asteraceae/*chemistry In Vitro Techniques Microbial Sensitivity Tests/methods Molecular Structure Oils, Volatile/chemistry/isolation & purification/*pharmacology Plant Extracts/chemist;" |
| Notes: | "MedlinePerez-Vasquez, Araceli Capella, Santiago Linares, Edelmira Bye, Robert Angeles-Lopez, Guadalupe Mata, Rachel eng Research Support, Non-U.S. Gov't England 2011/03/16 J Pharm Pharmacol. 2011 Apr; 63(4):579-86. doi: 10.1111/j.2042-7158.2010.01243.x. Epub 2011 Mar 1" |
