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Talanta

Title:		Development and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines
Author(s):		Paula Barros E; Moreira N; Elias Pereira G; Leite SG; Moraes Rezende C; Guedes de Pinho P;
Address:		"Instituto de Quimica, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira Ramos, 149, Bloco A, Laboratorio 626 A, 21941-909, Cidade Universitaria, Rio de Janeiro, Brazil. elisabete@eq.ufrj.br"
Journal Title:		Talanta
Year:		2012
Volume:		20120908
Issue:
Page Number:		177 - 186
DOI:		10.1016/j.talanta.2012.08.028
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, norisoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time, and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5 ml of wine with 2g NaCl at 45 degrees C during 5 min, and subsequent extraction of 30 min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile composition of different white wines"
Keywords:		*Automation Gas Chromatography-Mass Spectrometry/*methods Limit of Detection Reproducibility of Results Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis Wine/*analysis;
Notes:		"MedlinePaula Barros, Elisabete Moreira, Nathalie Elias Pereira, Giuliano Leite, Selma Gomes Ferreira Moraes Rezende, Claudia Guedes de Pinho, Paula eng Research Support, Non-U.S. Gov't Validation Study Netherlands 2012/11/20 Talanta. 2012 Nov 15; 101:177-86. doi: 10.1016/j.talanta.2012.08.028. Epub 2012 Sep 8"