| Title: | Detection of Volatile Compounds Emitted by Bacteria in Wounds Using Gas Sensors |
| Author(s): | Salinas Alvarez C; Sierra-Sosa D; Garcia-Zapirain B; Yoder-Himes D; Elmaghraby A; |
| Address: | "eVIDA Research Group, University of Deusto, 48007 Bilbao, Spain. carlosalinasal@gmail.com. Department of Computer Engineering and Computer Science (CECS), University of Louisville, Louisville, KY 40292, USA. d.sierrasosa@louisville.edu. eVIDA Research Group, University of Deusto, 48007 Bilbao, Spain. mbgarciazapi@deusto.es. Department of Biology, University of Louisville, Louisville, KY 40292, USA. deborah.yoder-himes@louisville.edu. Department of Computer Engineering and Computer Science (CECS), University of Louisville, Louisville, KY 40292, USA. adel@louisville.edu" |
| ISSN/ISBN: | 1424-8220 (Electronic) 1424-8220 (Linking) |
| Abstract: | "In this paper we analyze an experiment for the use of low-cost gas sensors intended to detect bacteria in wounds using a non-intrusive technique. Seven different genera/species of microbes tend to be present in most wound infections. Detection of these bacteria usually requires sample and laboratory testing which is costly, inconvenient and time-consuming. The validation processes for these sensors with nineteen types of microbes (1 Candida, 2 Enterococcus, 6 Staphylococcus, 1 Aeromonas, 1 Micrococcus, 2 E. coli and 6 Pseudomonas) are presented here, in which four sensors were evaluated: TGS-826 used for ammonia and amines, MQ-3 used for alcohol detection, MQ-135 for CO(2) and MQ-138 for acetone detection. Validation was undertaken by studying the behavior of the sensors at different distances and gas concentrations. Preliminary results with liquid cultures of 10(8) CFU/mL and solid cultures of 10(8) CFU/cm(2) of the 6 Pseudomonas aeruginosa strains revealed that the four gas sensors showed a response at a height of 5 mm. The ammonia detection response of the TGS-826 to Pseudomonas showed the highest responses for the experimental samples over the background signals, with a difference between the values ?ªn?ªnof up to 60 units in the solid samples and the most consistent and constant values. This could suggest that this sensor is a good detector of Pseudomonas aeruginosa, and the recording made of its values ?ªn?ªncould be indicative of the detection of this species. All the species revealed similar CO(2) emission and a high response rate with acetone for Micrococcus, Aeromonas and Staphylococcus" |
| Keywords: | Alcohols/analysis Ammonia/analysis Candida/chemistry/metabolism Escherichia coli/chemistry/metabolism Gases/*analysis Humans Pseudomonas aeruginosa/chemistry/metabolism Volatile Organic Compounds/analysis/*chemistry Wound Infection/*diagnosis/microbiology; |
| Notes: | "MedlineSalinas Alvarez, Carlos Sierra-Sosa, Daniel Garcia-Zapirain, Begonya Yoder-Himes, Deborah Elmaghraby, Adel eng Switzerland 2019/03/31 Sensors (Basel). 2019 Mar 28; 19(7):1523. doi: 10.3390/s19071523" |
