| Title: | Cascading Effects of Root Microbial Symbiosis on the Development and Metabolome of the Insect Herbivore Manduca sexta L |
| Author(s): | Papantoniou D; Vergara F; Weinhold A; Quijano T; Khakimov B; Pattison DI; Bak S; van Dam NM; Martinez-Medina A; |
| Address: | "German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Puschstrasse 4, 04103 Leipzig, Germany. Institute of Biodiversity, Friedrich-Schiller Universitat Jena, Dornburger Str. 159, 07743 Jena, Germany. Departamento de Ecologia Tropical, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan, Apartado Postal 4-116, Itzimna 97000, Mexico. Department of Food Science, University of Copenhagen Rolighedsvej 26, 1958 Frederiksberg C, Denmark. Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark. Plant-Microorganism Interaction, Institute of Natural Resources and Agrobiology of Salamanca, 37008 Salamanca, Spain" |
| ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
| Abstract: | "Root mutualistic microbes can modulate the production of plant secondary metabolites affecting plant-herbivore interactions. Still, the main mechanisms underlying the impact of root mutualists on herbivore performance remain ambiguous. In particular, little is known about how changes in the plant metabolome induced by root mutualists affect the insect metabolome and post-larval development. By using bioassays with tomato plants (Solanum lycopersicum), we analyzed the impact of the arbuscular mycorrhizal fungus Rhizophagus irregularis and the growth-promoting fungus Trichoderma harzianum on the plant interaction with the specialist insect herbivore Manduca sexta. We found that root colonization by the mutualistic microbes impaired insect development, including metamorphosis. By using untargeted metabolomics, we found that root colonization by the mutualistic microbes altered the secondary metabolism of tomato shoots, leading to enhanced levels of steroidal glycoalkaloids. Untargeted metabolomics further revealed that root colonization by the mutualists affected the metabolome of the herbivore, leading to an enhanced accumulation of steroidal glycoalkaloids and altered patterns of fatty acid amides and carnitine-derived metabolites. Our results indicate that the changes in the shoot metabolome triggered by root mutualistic microbes can cascade up altering the metabolome of the insects feeding on the colonized plants, thus affecting the insect development" |
| Keywords: | LC-qToF-MS Manduca sexta Solanum lycopersicum Trichoderma arbuscular mycorrhizal fungi metamorphosis; |
| Notes: | "PubMed-not-MEDLINEPapantoniou, Dimitra Vergara, Fredd Weinhold, Alexander Quijano, Teresa Khakimov, Bekzod Pattison, David I Bak, Soren van Dam, Nicole M Martinez-Medina, Ainhoa eng 765290/Horizon 2020 Framework Programme/ Switzerland 2021/11/26 Metabolites. 2021 Oct 25; 11(11):731. doi: 10.3390/metabo11110731" |
