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Data Brief

Title:		Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
Author(s):		Paille A; Charton R; Muguet A; Griesenbeck J; Smerdon MJ; Conconi A;
Address:		"Departement de Microbiologie et Infectiologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada. Institut fur Biochemie, Genetik und Mikrobiologie, Universitat Regensburg, 93053 Regensburg, Germany. Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, United States"
Journal Title:		Data Brief
Year:		2019
Volume:		20190531
Issue:
Page Number:		104083 -
DOI:		10.1016/j.dib.2019.104083
ISSN/ISBN:		2352-3409 (Electronic) 2352-3409 (Linking)
Abstract:		"The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150-200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (active/open), the other half is not transcribed and is assembled in regular arrays of nucleosomes (inactive/closed). It is proposed that RNA polymerase-I (RNAPI) transcription-elongation removes nucleosomes from closed rRNA genes (opening), and that soon after DNA replication there is deposition of nucleosomes on the open rRNA genes (closing). In G1 arrested cells, nearly all rRNA genes are depleted of nucleosomes, but most of them are not transcribed (inactive/open). In relation to the research article by Charton et al. (Mutat. Res.), the data presented here are on the hydroxyurea concentration-dependent inhibition of yeast culture growth, on cell cycle arrest before completion of genome replication, and on the opening of rRNA gene chromatin. As comparison, data are presented for yeast arrested in the G1-phase of the cell cycle by the pheromone alpha-factor"
Keywords:		Alpha-factor Chromatin Hydroxyurea Psoralen crosslinking RNA polymerase I rRNA genes;
Notes:		"PubMed-not-MEDLINEPaille, Audrey Charton, Romain Muguet, Alexia Griesenbeck, Joachim Smerdon, Michael J Conconi, Antonio eng R01 ES002614/ES/NIEHS NIH HHS/ R37 ES002614/ES/NIEHS NIH HHS/ Netherlands 2019/07/12 Data Brief. 2019 May 31; 25:104083. doi: 10.1016/j.dib.2019.104083. eCollection 2019 Aug"