| Title: | Molecular characterization of Saccharomyces cerevisiae alpha-pheromone by mass spectrometry-based peptidomics |
| Author(s): | Bener Aksam E; Pinkse MW; Verhaert PD; |
| Address: | "Department of Biotechnology, Kluyver Laboratory & Netherlands Proteomics Centre, Delft University of Technology, Delft, The Netherlands" |
| ISSN/ISBN: | 1567-1364 (Electronic) 1567-1356 (Linking) |
| Abstract: | "Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast alpha-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of alpha-pheromone (MFalpha and MFalpha') detectable and identifiable straight from WT supernatants. In addition to the mature intact alpha-pheromones, also post-translationally modified alpha-pheromone peptides and fragments thereof are found to be present in the culture medium. This molecular analytical technique is complementary to the recently described quantitation method by Rogers et al. (2012, FEMS Yeast Res. 12:668) based on ELISA" |
| Keywords: | Culture Media/*chemistry Peptides/*chemistry/*metabolism Pheromones/*chemistry/*metabolism Saccharomyces/*metabolism Tandem Mass Spectrometry; |
| Notes: | "MedlineBener Aksam, Eda Pinkse, Martijn W H Verhaert, Peter D E M eng Research Support, Non-U.S. Gov't England 2013/02/02 FEMS Yeast Res. 2013 May; 13(3):350-3. doi: 10.1111/1567-1364.12032. Epub 2013 Mar 11" |
