| Title: | "Mediation of Potato-Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compounds" |
| Author(s): | Ochola J; Cortada L; Ng'ang'a M; Hassanali A; Coyne D; Torto B; |
| Address: | "Behavioural and Chemical Ecology Unit, International Centre of Insect Physiology and Ecology, Nairobi, Kenya. Department of Chemistry, Kenyatta University, Nairobi, Kenya. Nematology Research Unit, Department of Biology, Ghent University, Ghent, Belgium. International Institute of Tropical Agriculture, Nairobi, Kenya" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids alpha-solanine and alpha-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors alpha-chaconine and alpha-solanine stimulated the highest number of eggs to hatch, approximately 47 and approximately 42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, approximately 60% of the hatched juveniles failed to emerge from the cyst, which was compound- and concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest" |
| Keywords: | Globodera spp.hatching factors potato root exudates semiochemicals steroidal glycoalkaloids; |
| Notes: | "PubMed-not-MEDLINEOchola, Juliet Cortada, Laura Ng'ang'a, Margaret Hassanali, Ahmed Coyne, Danny Torto, Baldwyn eng Switzerland 2020/06/27 Front Plant Sci. 2020 Jun 10; 11:649. doi: 10.3389/fpls.2020.00649. eCollection 2020" |
