| Title: | Leaf Endoplasmic Reticulum Bodies Identified in Arabidopsis Rosette Leaves Are Involved in Defense against Herbivory |
| Author(s): | Nakazaki A; Yamada K; Kunieda T; Sugiyama R; Hirai MY; Tamura K; Hara-Nishimura I; Shimada T; |
| Address: | "Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Malopolska Center of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland. Faculty of Science and Engineering, Konan University, Kobe 658-8501, Japan. RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan. Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan tshimada@gr.bot.kyoto-u.ac.jp" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "ER bodies are endoplasmic reticulum (ER)-derived organelles specific to the order Brassicales and are thought to function in plant defense against insects and pathogens. ER bodies are generally classified into two types: constitutive ER bodies in the epidermal cells of seedlings, and wound-inducible ER bodies in rosette leaves. Herein, we reveal a third type of ER body found in Arabidopsis (Arabidopsis thaliana) rosette leaves and designate them 'leaf ERbodies' (L-ER bodies). L-ER bodies constitutively occurred in specific cells of the rosette leaves: marginal cells, epidermal cells covering the midrib, and giant pavement cells. The distribution of L-ER bodies was closely associated with the expression profile of the basic helix-loop-helix transcription factor NAI1, which is responsible for constitutive ER-body formation. L-ER bodies were seldom observed in nai1 mutant leaves, indicating that NAI1 is involved in L-ER body formation. Confocal imaging analysis revealed that L-ER bodies accumulated two types of beta-glucosidases: PYK10, the constitutive ER-body beta-glucosidase; and BETA-GLUCOSIDASE18 (BGLU18), the wound-inducible ER-body beta-glucosidase. Combined with the absence of L-ER bodies in the bglu18 pyk10 mutant, these results indicate that BGLU18 and PYK10 are the major components of L-ER bodies. A subsequent feeding assay with the terrestrial isopod Armadillidium vulgare revealed that bglu18 pyk10 leaves were severely damaged as a result of herbivory. In addition, the bglu18 pyk10 mutant was defective in the hydrolysis of 4-methoxyindol-3-ylmethyl glucosinolate These results suggest that L-ER bodies are involved in the production of defensive compound(s) from 4-methoxyindol-3-ylmethyl glucosinolate that protect Arabidopsis leaves against herbivory attack" |
| Keywords: | Arabidopsis/*immunology/metabolism Arabidopsis Proteins/genetics/metabolism/physiology Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics/metabolism/physiology Endoplasmic Reticulum/metabolism/*physiology *Herbivory Plant Leaves/immunolo; |
| Notes: | "MedlineNakazaki, Akiko Yamada, Kenji Kunieda, Tadashi Sugiyama, Ryosuke Hirai, Masami Yokota Tamura, Kentaro Hara-Nishimura, Ikuko Shimada, Tomoo eng Research Support, Non-U.S. Gov't 2019/01/31 Plant Physiol. 2019 Apr; 179(4):1515-1524. doi: 10.1104/pp.18.00984. Epub 2019 Jan 29" |
