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Talanta

Title:		In vivo quantification of volatile organoselenium compounds released by bacteria exposed to selenium with HS-SPME-GC-MS. Effect of selenite and selenium nanoparticles
Author(s):		Moreno-Martin G; Sanz-Landaluze J; Leon-Gonzalez ME; Madrid Y;
Address:		"Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad Complutense de Madrid, 28040, Madrid, Spain. Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad Complutense de Madrid, 28040, Madrid, Spain. Electronic address: leongon@ucm.es. Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad Complutense de Madrid, 28040, Madrid, Spain. Electronic address: ymadrid@quim.ucm.es"
Journal Title:		Talanta
Year:		2021
Volume:		20201118
Issue:
Page Number:		121907 -
DOI:		10.1016/j.talanta.2020.121907
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"Quantification of volatile organoselenium species released by Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), after their growth in the presence of 1 and 2 mg Se.L(-1) as both selenite and chitosan-modified selenium nanoparticles (Ch-SeNPs), was achieved by the application of a method based on headspace solid-phase microextraction (HS-SPME) and in-fiber internal standardization, combined with gas chromatography coupled to mass spectrometry (GC-MS). This method consisted of an initial extraction of the released volatile organoselenium compounds on the SPME fiber, followed by the extraction of internal standard (IS), deuterated dimethyl sulfide (d(6)-DMS), on the same fiber before its desorption at the injection port of GC-MS. The results showed that the biotransformation of selenite and Ch-SeNPs into volatile organoselenium compounds was dependent on both the type of bacterial species and the chemical form of selenium (Se) administered. In this sense, E. coli was able to biotransform both selenite and Ch-SeNPs into dimethylselenium (DMSe) and dimethyldiselenium (DMDSe) while S. aureus, biotransformed selenite into DMSe and DMDSe and, Ch-SeNPs only into DMDSe. Additionally, the formation of a volatile mixed sulfur/selenium compound, dimethyl selenenyl sulfide (DMSeS), from Se in nanoparticulated form has been detected for the first time"
Keywords:		Escherichia coli Gas Chromatography-Mass Spectrometry *Nanoparticles *Organoselenium Compounds Selenious Acid *Selenium Solid Phase Microextraction Staphylococcus aureus *Volatile Organic Compounds/analysis Bacteria metabolisms In vivo headspace solid-pha;
Notes:		"MedlineMoreno-Martin, Gustavo Sanz-Landaluze, Jon Leon-Gonzalez, Maria Eugenia Madrid, Yolanda eng Netherlands 2021/01/01 Talanta. 2021 Mar 1; 224:121907. doi: 10.1016/j.talanta.2020.121907. Epub 2020 Nov 18"