| Title: | Improved monitoring of cryptogam gas-exchange and volatile emissions during desiccation-rehydration cycles with a within-chamber hydration method |
| Author(s): | Morales-Sanchez JA; Mark K; Talts E; Rasulov B; Niinemets U; |
| Address: | "Chair of Crop Science and Plant Biology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 5, Tartu 51006, Estonia. Electronic address: jose.morales@emu.ee. Chair of Crop Science and Plant Biology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 5, Tartu 51006, Estonia. Chair of Crop Science and Plant Biology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 5, Tartu 51006, Estonia; Estonian Academy of Sciences, Kohtu 6, Tallinn 10130, Estonia" |
| DOI: | 10.1016/j.plantsci.2023.111745 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "Desiccation-rehydration studies in cryptogams constitute an important tool to understand the relation of key physiological traits with species stress tolerance and environmental adaptability. Real-time monitoring of responses has been limited by the design of commercial or custom measuring cuvettes and difficulties in experimental manipulation. We developed a within-chamber rehydration method that allows to rewater the samples rapidly, without the need to open the chamber and take out the sample for manual rehydration by the investigator. Data is collected in real-time and simultaneously with an infrared gas-analyzer (LICOR-7000), a chlorophyll fluorometer (Maxi Imaging-PAM) and a proton transfer reaction time-of-flight mass-spectrometer (PTR-TOF-MS) for volatile organic compound emissions. The system was tested on four cryptogam species with contrasting ecological distributions. No major errors or kinetics disruptions were found during system testing and measurements. Our within-chamber rehydration method improved accuracy, as measurement periods were not lacking, and repeatability of the protocol by reducing error variance in sample manipulation. This method provides an improved technique to conduct desiccation-rehydration measurements, contributing to the standardization and accuracy of current existing methodologies. A close real-time and simultaneous monitoring of photosynthesis, chlorophyll fluorescence and volatile organic compound emission data, offers a novel perspective in the analysis of the cryptogam stress responses that is yet to be fully explored" |
| Keywords: | *Desiccation *Volatile Organic Compounds/analysis Photosynthesis/physiology Chlorophyll Fluid Therapy Bryophytes Chlorophyll fluorescence Desiccation Lichens Photosynthesis Rehydration Volatile organic compounds; |
| Notes: | "MedlineMorales-Sanchez, Jose Angel Mark, Kristiina Talts, Eero Rasulov, Bakhtier Niinemets, Ulo eng Ireland 2023/05/28 Plant Sci. 2023 Aug; 333:111745. doi: 10.1016/j.plantsci.2023.111745. Epub 2023 May 25" |
