| Title: | Metabolic Profiling of VOCs Emitted by Bacteria Isolated from Pressure Ulcers and Treated with Different Concentrations of Bio-AgNPs |
| Author(s): | Monedeiro F; Railean-Plugaru V; Monedeiro-Milanowski M; Pomastowski P; Buszewski B; |
| Address: | "Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Torun, 4 Wilenska St., 87-100 Torun, Poland. Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, 7 Gagarina St., 87-100 Torun, Poland" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Considering the advent of antibiotic resistance, the study of bacterial metabolic behavior stimulated by novel antimicrobial agents becomes a relevant tool to elucidate involved adaptive pathways. Profiling of volatile metabolites was performed to monitor alterations of bacterial metabolism induced by biosynthesized silver nanoparticles (bio-AgNPs). Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis were isolated from pressure ulcers, and their cultures were prepared in the presence/absence of bio-AgNPs at 12.5, 25 and 50 microg mL(-1). Headspace solid phase microextraction associated to gas chromatography-mass spectrometry was the employed analytical platform. At the lower concentration level, the agent promoted positive modulation of products of fermentation routes and bioactive volatiles, indicating an attempt of bacteria to adapt to an ongoing suppression of cellular respiration. Augmented response of aldehydes and other possible products of lipid oxidative cleavage was noticed for increasing levels of bio-AgNPs. The greatest concentration of agent caused a reduction of 44 to 80% in the variety of compounds found in the control samples. Pathway analysis indicated overall inhibition of amino acids and fatty acids routes. The present assessment may provide a deeper understanding of molecular mechanisms of bio-AgNPs and how the metabolic response of bacteria is untangled" |
| Keywords: | "Anti-Bacterial Agents/therapeutic use Bacteria/*drug effects/isolation & purification/*metabolism Drug Resistance, Bacterial Enterococcus faecalis/drug effects/isolation & purification/metabolism Escherichia coli/drug effects/isolation & purification/meta;" |
| Notes: | "MedlineMonedeiro, Fernanda Railean-Plugaru, Viorica Monedeiro-Milanowski, Maciej Pomastowski, Pawel Buszewski, Boguslaw eng FNP 4.4 PO IR 2014-2020/Foundation for Polish Science/ Switzerland 2021/05/06 Int J Mol Sci. 2021 Apr 29; 22(9):4696. doi: 10.3390/ijms22094696" |
