« Previous Abstract"Involvement of protein sulfhydryls in the trigger reaction of rhodotorucine A, a farnesyl peptide mating pheromone of Rhodosporidium toruloides"    Next Abstract"Cell communication, cell union, and initiation of meiosis in ciliate conjugation" »

Biochem Biophys Res Commun

Title:		"Inhibition of membrane Ca2+-ATPase in vitro by mating pheromone in Rhodosporidium toruloides, a heterobasidiomycetous yeast"
Author(s):		Miyakawa T; Tachikawa T; Jeong YK; Tsuchiya E; Fukui S;
Address:
Journal Title:		Biochem Biophys Res Commun
Year:		1987
Volume:		143
Issue:		3
Page Number:		893 - 900
DOI:		10.1016/0006-291x(87)90333-0
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"Direct addition of physiological concentrations of rhodotorucine A, a lipopeptide mating pheromone of Rhodosporidium toruloides, to the particulate fraction of the target cell strongly inhibited Ca2+-ATPase activity. The pheromone effect was mating-type specific. Membrane Ca2+-ATPase solubilized by a nonionic detergent and further purified by calmodulin-affinity chromatography was also inhibited by the pheromone. Rhodotorucine A S-oxide, a biologically inactive analogue, had no effect on Ca2+-ATPase. The results suggested that the inhibition of membrane Ca2+-ATPase is a critical event in the signaling of mating pheromone and the inhibition of membrane Ca2+-pump could be responsible for the pheromone-induced rapid raise of intracellular Ca2+ concentration reported"
Keywords:		Basidiomycota/cytology/*drug effects/enzymology Calcium-Transporting ATPases/*antagonists & inhibitors Oligopeptides/*pharmacology Pheromones/pharmacology Subcellular Fractions/enzymology;
Notes:		"MedlineMiyakawa, T Tachikawa, T Jeong, Y K Tsuchiya, E Fukui, S eng 1987/03/30 Biochem Biophys Res Commun. 1987 Mar 30; 143(3):893-900. doi: 10.1016/0006-291x(87)90333-0"