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Arch Insect Biochem Physiol

Title:		Inga laurina trypsin inhibitor (ILTI) obstructs Spodoptera frugiperda trypsins expressed during adaptive mechanisms against plant protease inhibitors
Author(s):		Machado SW; de Oliveira CFR; Zerio NG; Parra JRP; Macedo MLR;
Address:		"Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas, Unidade de Tecnologia de Alimentos e da Saude Publica, Universidade Federal de Mato Grosso do Sul, Campo Grande, Brazil. Faculdade de Medicina, FAMED, Universidade Federal de Mato Grosso do Sul, Campo Grande, Brazil. Departamento de Entomologia e Acarologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, Brazil"
Journal Title:		Arch Insect Biochem Physiol
Year:		2017
Volume:		20170629
Issue:		4
Page Number:		-
DOI:		10.1002/arch.21393
ISSN/ISBN:		1520-6327 (Electronic) 0739-4462 (Linking)
Abstract:		"Plant protease inhibitors (PIs) are elements of a common plant defense mechanism induced in response to herbivores. The fall armyworm, Spodoptera frugiperda, a highly polyphagous lepidopteran pest, responds to various PIs in its diet by expressing genes encoding trypsins. This raises the question of whether the PI-induced trypsins are also inhibited by other PIs, which we posed as the hypothesis that Inga laurina trypsin inhibitor (ILTI) inhibits PI-induced trypsins in S. frugiperda. In the process of testing our hypothesis, we compared its properties with those of selected PIs, soybean Kunitz trypsin inhibitor (SKTI), Inga vera trypsin inhibitor (IVTI), Adenanthera pavonina trypsin inhibitor (ApTI), and Entada acaciifolia trypsin inhibitor (EATI). We report that ILTI is more effective in inhibiting the induced S. frugiperda trypsins than SKTI and the other PIs, which supports our hypothesis. ILTI may be more appropriate than SKTI for studies regarding adaptive mechanisms to dietary PIs"
Keywords:		"Animals Fabaceae/*chemistry *Insect Control Insect Proteins/*antagonists & inhibitors/isolation & purification Larva Spodoptera/*enzymology Trypsin Inhibitor, Kunitz Soybean Trypsin Inhibitors/*chemistry/isolation & purification Inga laurina Spodoptera fr;"
Notes:		"MedlineMachado, Suzy Wider de Oliveira, Caio Fernando Ramalho Zerio, Neide Graciano Parra, Jose Roberto Postali Macedo, Maria Ligia Rodrigues eng 2017/07/01 Arch Insect Biochem Physiol. 2017 Aug; 95(4). doi: 10.1002/arch.21393. Epub 2017 Jun 29"