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Chin J Biotechnol

Title:		Expression and secretion of alpha-amylase and glucoamylase in Saccharomyces cerevisiae
Author(s):		Luo J; He M; Li W; Zhang T;
Address:		"Biotechnology Research Center, Zhongshan University, Guangzhou, China"
Journal Title:		Chin J Biotechnol
Year:		1994
Volume:		10
Issue:		4
Page Number:		241 - 248
DOI:
ISSN/ISBN:		1042-749X (Print) 1042-749X (Linking)
Abstract:		alpha-Amylase genes of Bacillus licheniformis and glucoamylase cDNA of Aspergillus niger were ligated to a E. coli-yeast shuttle vector. The resultant plasmid was used to transform Saccharomyces cerevisiae to construct starch-degrading yeast strain. The results of enzyme activity assay and enzyme property analysis show that alpha-amylase and glucoamylase genes have been expressed simultaneously in yeast under the control of promoters and terminators of yeast MF-alpha 1 factor and PGK genes and over 99% of enzyme activities were secreted to the medium. The engineered yeast strain hydrolyses 97% of the starch (10%) in the medium after 6 days. The recombinant plasmid exists stably in yeast
Keywords:		"Aspergillus niger/enzymology/genetics Bacillus/enzymology/genetics Bacterial Proteins/biosynthesis/genetics Cloning, Molecular Fungal Proteins/biosynthesis/genetics Glucan 1, 4-alpha-Glucosidase/*biosynthesis/genetics Mating Factor Peptides/genetics Phosph;"
Notes:		"MedlineLuo, J He, M Li, W Zhang, T eng 1994/01/01 Chin J Biotechnol. 1994; 10(4):241-8"