| Title: | Identification of cryptic species within liverwort Conocephalum conicum based on the volatile components |
| Author(s): | Ludwiczuk A; Odrzykoski IJ; Asakawa Y; |
| Address: | "Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki Str., 20-093 Lublin, Poland. Electronic address: aludwiczuk@pharmacognosy.org" |
| DOI: | 10.1016/j.phytochem.2013.06.011 |
| ISSN/ISBN: | 1873-3700 (Electronic) 0031-9422 (Linking) |
| Abstract: | "Cryptic speciation refers to cases where species are clearly genetically differentiated, but show little or no morphological differences. This study investigated whether volatile components can be used to distinguish the cryptic species within the Conocephalum conicum complex. Thirteen samples were included in this study representing four cryptic species (A, F, J, L) and the recently described Conocephalum salebrosum (formerly cryptic species S) detected in a worldwide collection. Results received from GC-MS analyses showed some differences between samples, indicating the existence of chemical polymorphism. It is evident from both statistical analyses (CA and PCA), that cryptic species L appears to be the most differentiated species, with the volatile components not significantly shared with the rest of the types. The most characteristic compound of this type is the sesquiterpene alcohol, conocephalenol. Distinction between other examined cryptic species were also indicated. However species F and J, as well as species A and C. salebrosum, share a great similarity in their volatile composition and are grouped together in both CA and PCA. Despite their similarities, we were able to find chemical markers which can describe each species. Cubebol is characteristic for C. salebrosum, while cryptic species A produces (E)-methylcinnamate. The presence of a large amount of the monoterpene hydrocarbon, sabinene, is characteristic for cryptic species J. In one of the analyzed specimens belonging to the cryptic species F, a large amount of cyclocolorenone was detected. Chemical differentiation was correlated with the geographical distribution of the analyzed samples" |
| Keywords: | Bicyclic Monoterpenes Hepatophyta/*chemistry/genetics Monoterpenes/*analysis Sesquiterpenes/*analysis Species Specificity Volatile Organic Compounds/*analysis Ca Conocephalaceae Conocephalum conicum Conocephalum salebrosum Cryptic species Liverworts Pca T; |
| Notes: | "MedlineLudwiczuk, Agnieszka Odrzykoski, Ireneusz J Asakawa, Yoshinori eng Research Support, Non-U.S. Gov't England 2013/07/10 Phytochemistry. 2013 Nov; 95:234-41. doi: 10.1016/j.phytochem.2013.06.011. Epub 2013 Jul 5" |
