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Int J Mol Sci

Title:		Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-alpha-Pinene Tolerance in Dendroctonus armandi
Author(s):		Liu B; Tang M; Chen H;
Address:		"College of Forestry, Northwest A&F University, Xianyang 712100, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China"
Journal Title:		Int J Mol Sci
Year:		2022
Volume:		20220930
Issue:		19
Page Number:		-
DOI:		10.3390/ijms231911578
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Bark beetles mainly rely on detoxification enzymes to resist the host tree's defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (DaCYP4BQ1) is associated with the response of (+)-alpha-pinene in Dendroctonus armandi. However, the regulatory mechanism of this P450 gene response to (+)-alpha-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that CYP4BQ1 was highly expressed in adult and larval stages of D. armandi, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of CYP4BQ1 significantly increased after exposure to (+)-alpha-pinene, and depletion of it decreased the tolerance of adults to (+)-alpha-pinene. In addition, (+)-alpha-pinene treatment induced the expression of the transcription factors cap 'n' collar isoform C (CncC) and its binding factor muscle aponeurosis fibromatosis (Maf), elevated the level of hydrogen peroxide (H(2)O(2)), and increased the activities of antioxidant enzymes. Silencing CncC suppressed CYP4BQ1 expression and enhanced the susceptibility of beetles to (+)-alpha-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H(2)O(2), suppressed the expression of CncC, Maf, and CYP4BQ1 and led to decreased tolerance of adults to (+)-alpha-pinene. In contrast, ingestion of the CncC agonist curcumin elevated CYP4BQ1 expression and enhanced (+)-alpha-pinene tolerance. The results demonstrate that, in D. armandi, (+)-alpha-pinene induces CYP4BQ1 via activation of the ROS/CncC signaling pathway"
Keywords:		Acetylcysteine Animals Antioxidants Bicyclic Monoterpenes *Curcumin Cytochrome P-450 Enzyme System/genetics/metabolism Hydrogen Peroxide *Pesticides Pheromones Reactive Oxygen Species Signal Transduction Terpenes Transcription Factors/genetics CncC pathwa;
Notes:		"MedlineLiu, Bin Tang, Ming Chen, Hui eng 31870636/National Natural Science Foundation of China/ Switzerland 2022/10/15 Int J Mol Sci. 2022 Sep 30; 23(19):11578. doi: 10.3390/ijms231911578"